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Ube2a (NM_019668) Mouse Untagged Clone
CAT＃: MC202831
Ube2a (untagged) - Mouse ubiquitin-conjugating enzyme E2A, RAD6 homolog (S. cerevisiae) (Ube2a), (10ug)
CNY 1,200.00
CNY 2,000.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | HHR6A; HR6A; Mhr6a |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC026053 sequence for NM_019668
CCACGCGTCCGGCGGCAGTGCCGTCCCTCTCGGCGCCTCCTCGGCTTCTCCGGCTTCCTCGGCTTCCTCG GGGACCGCGGACCCTGGTATATGCCCGACTGCTGAGCCCGCTAAAGCCATGTCGACCCCGGCCCGGCGCC GCCTCATGAGGGACTTCAAGAGGTTACAGGAAGATCCTCCGGCCGGAGTGAGCGGGGCTCCGTCGGAGAA CAACATAATGGTTTGGAATGCGGTCATTTTTGGGCCCGAAGGGACCCCGTTTGAGGATGGAACATTTAAG CTTACAATTGAATTCACTGAAGAGTATCCTAATAAGCCACCTACTGTTAGATTTGTCTCTAAGATGTTCC ATCCTAACGTCTATGCAGATGGTAGTATATGTCTGGATATACTTCAGAACCGTTGGAGTCCAACCTATGA TGTGTCTTCCATTTTAACGTCCATACAGTCTCTATTGGATGAACCTAATCCCAATAGTCCAGCAAACAGC CAGGCTGCCCAGCTGTACCAGGAGAACAAGCGGGAATATGAAAAGCGTGTTTCTGCAATAGTAGAACAAA GCTGGCGCGACTGTTGACTCAGGGTAGAGCAGAAGAAGAGGCTGGCCATAAGAAAAATGTATATTGATGC ATTTATCACCTTATTCCTAATTCATTACATTTACTTTATTAAAAACAAAATAGCTGTTGTGCTGTTTCTG TCTTCCCTTGGCAAGTTTTTCTTCCCCTTTCTGTCCTCCTGTGGAGCATCAGAGATATTATTTGAAATCC AATGTACTGTACCTGGGTTACTTGCAAACATTACAATTGCATATGTCCCTTTGTTGTATCTCATCTCCAG CCTCAAAGCAGTTATCAGGTTACTGTACTTTTTATACTAAGCTTTTAAGATAAAATATTATGTATAGTCT GGTGACCAGGATGTTATTTGTAACAAAATGATTGCTGCTGTGTTTCATCCTGGCCATTCCTTCCCTTGTA TTTGATTTGGCAAAGAATGTATTTAGAATATGACCTAAAGAAAACAGGAATACATTCATATAAACAATAT TTGAAAGGGGAAGGAAAGCTGAAAAACCCTATACTCATTTGTAAGCAATATGAACATGAACTACAAGATG GCACTTACTGGTAGCAAGGAGACTGCTAAAGGACCCAGCTAACAAACTGAAGCAATTTTAATAACTGACA CAGTCCACTTTGTGCGAGATATTCATAGTGTAAGCGCATTCTGTTTCGGAATTCTGGCCTTCAGAGTTGT TTTTTATAGAGGTGTAAGAACAGGACTAGACTTTTTTTTAAAGTTAGGAAAAACCGAAGATCCTCTATTC TCTTAAAGGAGCAGTGTTGCCCTAAATGAACTTTCCTTTTTATGATTCACGGAAGGGAGTGTATCTTGCA AACTTTTCCCATTCAAATCCTGAGTTAATGCTGCATGCTTTCAGTGTTTTGAGAACCTTCTATTGCCCAT ATCTGCAATTTTTTCTTTGAATATTTACACTTTTAAATATACAGTATGGGGCAAGGCTTGTAAATGTTTT GTCTAATATATTTTCATTGTTAATCTATTATGCATTTATAGCATTTCTAACTTTGCACAAGTAACCCATG TAAAAACTGTACATTTTCAAAAGTTGTAAATAAAAAAATAACCTTAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_019668 |
| Insert Size | 459 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC026053, AAH26053 |
| RefSeq Size | 1744 bp |
| RefSeq ORF | 459 bp |
| Locus ID | 22209 |
| UniProt ID | Q9Z255 |
| Gene Summary | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG201102 | Ube2a (tGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2A, RAD6 homolog (S |
CNY 2,850.00 |
|
| MR201102 | Ube2a (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2A, RAD6 homolog (S. cerevisiae) (Ube2a) |
CNY 1,200.00 |
|
| MR201102L3 | Lenti ORF clone of Ube2a (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2A, RAD6 homolog (S. cerevisiae) (Ube2a) |
CNY 4,750.00 |
|
| MR201102L4 | Lenti ORF clone of Ube2a (mGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2A, RAD6 homolog (S. cerevisiae) (Ube2a) |
CNY 4,750.00 |
