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Mcoln2 (NM_001005846) Mouse Untagged Clone
CAT＃: MC202191
Mcoln2 (untagged) - Mouse mucolipin 2 (Mcoln2), transcript variant 2, (10ug)
CNY 4,016.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 3300002C04Rik; AI549968; C86638; TRPML2 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC029847 sequence for NM_001005846
GAGATCAGAGCGCTGACTGGAAGGATCTGCGATGGCGTCTCTCGATTCTGAGGTGAGGGAAGAGTGTCTG AGGGAAGACCTGAAGTTCTACTTCATGAGTCCCTGTGAGAAGTACCGAGCCAGACGTCAGATCCCGTGGA AACTGGGCTTGCAGATATTGAAGATAGTCATGGTAACCACACAGCTTGTTCGGTTTGGCTTAAGTAACCA GTTGGTGGTGGCTTTCAAAGAAGATAACACTGTTGCTTTTAAGCACTTGTTTTTGAAAGGATTTTCTGGA GTTGATGAAGATGACTACAGCTGTAGCATATACACTCAGGAGAACACCTATGAGAGCATCTTTTTTGCTA TAAAGCAGTATCGTCATCTGAAGAATATTTCCCTGGCAACCCTTGGTTATGGAGAAAGTGAAGACAATAG AACTGGCTTGAAGGTCTGTAAGCAGCACTACAAGACAGGGGCCATGTTTTCTTCTAACGAGACCCTGAAC ATTGACAGTGACATTGAGACAGACTGCATTCACCTGGACCTTCAGGTGCTCACCACGGAACCCGAGGACT GGGCACAGACATCTTTCTTCAGGCTGGATTTTTATCGGCTCGTGCAAGTTGATATCTCCTTTGCCCTCAA AGGAATTGACCTACAGGCAGTTCATTCCCGAGAGATACCGGACTGTTACCTCTTTCAGAATACGATTACC TTCGACAACACAGCCCACAGTGGGAAAATCAAAATCTATCTCAACAGCGAGGCCAACATCGAAGAGTGTA AAAACATGAACATATCTGGGTCTACTCAGAGAAGCACTCACTACCTCCTGGTGTTTGATGTGTTCGTCAT TATGATCTGCCTGGCATCCCTTATCCTGTGCACGAGATCCATTGTTCTTGCTCTGAGGCTGAGGAAGAGA TTTCTAAACTTCTTCCTGGAGAAGTACAAACAGCGCGTGTGTGGCGCTGACCAGTGGGAATTCGTCAACG GATGGTACGTCCTGGTCACTATCAGCGACCTCATGACAATAATTGGATCCATATTGAAGATGGAAATCAA AGCCAAGAAGCTCACAAACTATGACGTGTGCAGCATCTTGCTCGGGACGTCGACGCTCTTTGTCTGGGTT GGAGTCATCAGATATCTGGGTTACTTTCAGACGTACAACGTGCTCATTTTAACCATGCAAGCCTCACTGC CCAAAGTTCTCCGGTTCTGTGCTTGCGCTGGCATGATCTACCTGGGGTACACCTTCTGCGGCTGGATTGT CTTAGGACCGTACCACGAGAAGTTTGAAAACCTGAACATAGTCGCTGAATGCCTGTTTTCTCTGGTCAAC GGCGATGACATGTTTGCCACGTTCGCTCAGATCCAGCAGAAGAGCATTCTGGTGTGGCTGTTCAGCCGCC TCTACCTGTATTCCTTTATCAGCCTATTCATTTACATGGTCCTCAGCCTCTTCATCGCGCTCATCACCGA CTCTTACCACACCATTAAGAAGTACCAGCAGCATGGGTTTCCTGAAACAGATCTGCAGAAATTCCTGAAG GAGAGCGGAAGCAAAGATGGGTACCAGAAGCAGCCATCGGCCTTGCTGTCCTGCCTCTGCTGTCTGAGGA GGAGAAGAAACAATGACCACTTGATTCTCATTGACTAAAACCTTTGGCTGATGTCAGTCAAGTCCAGATC CCTTTATGCTGAGGTCATTCAGAGAGACCCCAGAGGTAGCAGCAGTCGGCTCAAAGATTGTCCTGCTGAA TTGTGGAACCAGAAGGAGGGGTGTCCATGAGCTGCTGATCAGACCTGACATTCCAAAGTTACTTTTCATA GACATGGAGTGTGAAGAGTAAGCCTCTTGTGAGGCCAGCGTGGCAATGAAATTCACCAGGAGTCTGGTCG TTTGTGACATCATAATGTTGAGATGTAAAAAGAAATATTCCCACTTCAGAGTGTGTAAGCAATATTTAAA CCCAGATAAAGATGCATGCTTATTGAAAAAACAAAGATTTGTTTCAAAACGTGACACTTGGATTTGAAGA CATTTGTTAATTTATGGTCTGCACAGAAATAAATTTGCTTCCAAACCAAATTAAGACTTGAAGCTAGAGA GTGGTAAGCCACTCAGTTCGTGACTGGTTGGACTGTGAGCTTGCTTCTTCAGCAAGCACAACACCAGTGT CTGTTAGCACAGTCAGCGGGATGAAAGAGCACCTGGCTGTCCCCTCAGGTGAGCTCTCCCTGGACTGGAC ACATGATGAGTGAGTCACCCTAAGCTTGGAGTGGCCATCTGTTTCACAAGTCAGCTCCATTGTGGTGGGG ATTTGCCCTTGTCATGGAAGGAAGTGTAGATTTTTCATTAAATAAATTTATTTTCACGAAAAAAAAAAAA AAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_001005846 |
| Insert Size | 1617 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC029847, AAH29847 |
| RefSeq Size | 2390 bp |
| RefSeq ORF | 1617 bp |
| Locus ID | 68279 |
| UniProt ID | Q8K595 |
| Gene Summary | Nonselective cation channel probably playing a role in the regulation of membrane trafficking events. Acts as Ca(2+)-permeable cation channel with inwardly rectifying activity (PubMed:19763610). May activate ARF6 and be involved in the trafficking of GPI-anchored cargo proteins to the cell surface via the ARF6-regulated recycling pathway (By similarity). May play a role in immune processes. In adaptive immunity, TRPML2 and TRPML1 may play redundant roles in the function of the specialized lysosomes of B cells (PubMed:17050035). In the innate immune response, may play a role in the regulation of chemokine secretion and macrophage migration (PubMed:26432893). Through a possible and probably tissue-specific heteromerization with MCOLN1 may be at least in part involved in many lysosome-dependent cellular events.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has an alternate 5' exon and uses a downstream AUG start codon, as compared to variant 1. The encoded isoform 2 has a shorter N-terminus, as compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208605 | Mcoln2 (tGFP-tagged) - Mouse mucolipin 2 (Mcoln2), transcript variant 2 |
CNY 5,230.00 |
|
| MR208605 | Mcoln2 (Myc-DDK-tagged) - Mouse mucolipin 2 (Mcoln2), transcript variant 2 |
CNY 4,016.00 |
|
| MR208605L3 | Lenti ORF clone of Mcoln2 (Myc-DDK-tagged) - Mouse mucolipin 2 (Mcoln2), transcript variant 2 |
CNY 6,650.00 |
|
| MR208605L4 | Lenti ORF clone of Mcoln2 (mGFP-tagged) - Mouse mucolipin 2 (Mcoln2), transcript variant 2 |
CNY 6,650.00 |
