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Lefty1 (NM_010094) Mouse Untagged Clone
CAT＃: MC202041
Lefty1 (untagged) - Mouse left right determination factor 1 (Lefty1), (10ug)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI450052; Eba; Ebaf; Left; Leftb; lefty; lefty-; lefty-1; St; Stra3; Tgfb4 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC050221 sequence for NM_010094
CACACATCCAAGGCTCCTCTTCCCGGACAGCACCATGCCATTCCTGTGGCTCTGCTGGGCACTCTGGGCA CTGTCGCTGGTTAGCCTCAGGGAAGCCCTGACCGGAGAGCAGATCCTGGGCAGCCTGCTGCAACAGCTGC AGCTCGATCAACCGCCAGTCCTGGACAAGGCTGATGTGGAAGGGATGGTCATCCCCTCGCACGTGAGGAC TCAGTATGTGGCCCTGCTACAACACAGCCATGCCAGCCGCTCCCGAGGCAAGAGGTTCAGCCAGAACCTT CGAGAGGTGGCAGGCAGGTTCCTGGTGTCAGAGACCTCCACTCACCTGCTAGTGTTCGGAATGGAGCAGC GGCTGCCGCCTAACAGCGAGCTGGTGCAGGCTGTGCTGCGGCTGTTCCAGGAGCCTGTGCCCAGAACAGC TCTCCGGAGGCAAAAGAGGCTGTCCCCACACAGTGCCCGGGCTCGGGTCACCATTGAATGGCTGCGCTTC CGCGACGACGGCTCCAACCGCACTGCCCTTATCGATTCTAGGCTCGTGTCCATCCACGAGAGCGGCTGGA AGGCCTTCGACGTGACCGAGGCCGTGAACTTCTGGCAGCAGCTGAGCCGGCCGAGGCAGCCGCTGCTGCT CCAGGTGTCGGTGCAGAGGGAGCATCTGGGGCCGGGAACCTGGAGCTCACACAAGTTGGTTCGTTTCGCG GCGCAGGGGACGCCGGATGGCAAGGGGCAGGGCGAGCCACAGCTGGAGCTGCACACGCTGGACCTCAAGG ACTATGGAGCTCAAGGCAATTGTGACCCCGAGGCACCAGTGACTGAAGGCACCCGATGCTGTCGCCAGGA GATGTACCTGGACCTGCAGGGGATGAAGTGGGCCGAGAACTGGATCCTAGAACCGCCAGGGTTCCTGACA TATGAATGTGTGGGCAGCTGCCTGCAGCTACCGGAGTCCCTGACCAGCAGGTGGCCATTTCTGGGGCCTC GGCAGTGTGTCGCCTCAGAGATGACCTCCCTGCCCATGATTGTCAGCGTGAAGGAGGGAGGCAGGACCAG GCCTCAAGTGGTCAGCCTGCCCAACATGAGGGTGCAGACCTGTAGCTGCGCCTCAGATGGGGCGCTCATA CCCAGGAGGCTGCAGCCATAGGCGCGGGGTGTGGCTTCCCCAAGGATGTGCCTTTCATGCAAATCTGAAG TGCTCATTATACTGGGAGAGCTGGGGATTCTAACTCCCTAATGGGCAATCCCTGTGTGTGCTCTTTGCTT CCTCTGAAGTAGCCTCATCCCTAAATTTTTACCTTCGAGGAATGTGACTCGCTGGCCCCTGGAGGCGCTC TGACCCAGTGGTCTCTGTCCTTCATATTGTTCACTGCACTGTATGCGAAGCACTTACATGTATAGATACT GCAAACCAAGGACAGAATCCCCAATTGCCATTGTTCCCTTAATTTGTCGCTGAATCTGGGCTGAGTCCCA GTCTTGACTCTGGACCTAAGCCACAAGTTGGGCAAACATGTCCAACCTAGGCAATACTGGCTTTGCTAGA TGTGAATAAAATATGCTTTGTTTTGTAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_010094 |
| Insert Size | 1107 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC050221, AAH50221 |
| RefSeq Size | 1582 bp |
| RefSeq ORF | 1107 bp |
| Locus ID | 13590 |
| UniProt ID | Q64280 |
| Gene Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. Mice lacking a functional copy of this gene exhibit embryonic lethality and defects in left-right patterning. [provided by RefSeq, Aug 2016] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG205659 | Lefty1 (tGFP-tagged) - Mouse left right determination factor 1 (Lefty1) |
CNY 3,140.00 |
|
| MR205659 | Lefty1 (Myc-DDK-tagged) - Mouse left right determination factor 1 (Lefty1) |
CNY 3,656.00 |
|
| MR205659L3 | Lenti ORF clone of Lefty1 (Myc-DDK-tagged) - Mouse left right determination factor 1 (Lefty1) |
CNY 4,750.00 |
|
| MR205659L4 | Lenti ORF clone of Lefty1 (mGFP-tagged) - Mouse left right determination factor 1 (Lefty1) |
CNY 4,750.00 |
