Dtd1 (NM_025314) Mouse Untagged Clone

CAT#: MC201830

Dtd1 (untagged) - Mouse D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae) (Dtd1), nuclear gene encoding mitochondrial protein, (10ug)



  "NM_025314" in other vectors (4)

CNY 2,400.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 0610006H08Rik; DTD; DUE-B; Hars2
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC026537 sequence for NM_025314
GTCGAGGAGCCACCGGCGGAGTCGCAAGGTCTGACCACACAGCAAATTCCAGTCGCTACAGCCGCCGCCA TGAAGGCCGTGGTGCAGCGCGTCACTCGGGCTAGCGTCACAGTTGGAGGAGAGCAGATAAGCGCCATCGG ACGGGGCATCTGTGTCTTGCTGGGTATTTCCATGGAAGACTCACAGAAGGAACTGGAACACATGGTTCGA AAGATTTTAAATCTGCGTGTGTTTGAGGACGAGAGTGGGAAGCACTGGTCCAAGAGCGTGATGGATAAGG AGTACGAGGTGCTGTGTGTCAGCCAGTTCACCCTCCAGTGCGTCCTCAAGGGGAACAAGCCCGACTTCCA CCTGGCCATGCCCACAGAGCAGGCAGAGAGCTTCTACAACAGCTTCCTGGAGCAGCTTCGCAAGAGCTAC AGACCAGAGCTTATCAGAGATGGCAAGTTTGGTGCCTACATGCAGGTGCACATTCAGAACGATGGGCCTG TGACTATAGAGCTGGAGTCCCCTGCTCCTGGAGCTGCTAGCTCTGACCCAAAGCAGCTGTCAAAGCTGGA GAAACAGCAGCAGAGAAAAGAAAAGACCAGAGCCAAAGGCCCTTCTGAGTCAAGCAAAGAACGGAATGCA CCCCGCAAAGAAGACCGCAGCGCCAGCAGCGGGGCAGAGGGTGACGTGTCTTCTGAGCGGGAGCCATAGA TGCGGATGCAGGAGCTCAGCAAATTACCATTGGGCAGAATCGGATCCTGAAAAATCAAGATGCTAAAGCC CCTACACTGCTTTAAGAATTCGGGACTCAAATATGAAAAGAAAATCAGAAATAAGACCCTGGGAACCTGA ATATCCCTGCAGAAAAACACCAAAGGACCATTTTATTTTGTGTATTGCTGTGGTCTGATTGGGGTAGTGG GTGCTGTGGGATGGGAGAGTGCTTGTGGGTTCACACCCATGCCCTCCCCATGGAGAGGGGGCCATTGGTT TTCTTGCTTTCTACCATGTCTTGGCTTGCTTTTGGAACAGGCTGGCCTAGCATCATCTGTCCTCATCTGT GGTGTACTGTGAGTCTGTGATCCATCTCCAGCACATGCCTGCTGTCCACCATTTCCATCATTGAGTCACA AGACAGGGCTGGTGTTAGGCAACCAATAATAACATATTGTATGAGACTTTGCATCTTGTACATTTTCTCT TCTTTTCTAAAAATAAATAATAAAACCATAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_025314
Insert Size 630 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC026537, AAH26537
RefSeq Size 1239 bp
RefSeq ORF 630 bp
Locus ID 66044
UniProt ID Q9DD18
Gene Summary An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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