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Pphln1 (NM_146062) Mouse Untagged Clone
CAT＃: MC201506
Pphln1 (untagged) - Mouse periphilin 1 (Pphln1), transcript variant 1, (10ug)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | CR; HSPC206; HSPC232 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC031486 sequence for NM_146062
GGCGGCTGAGGCGGCGGCGACAGAGGCGGCGGCGACAGAGGCGGAGGCTCAGCAGTGGGACGTAGTGGCT TCCACAAGAGATGAAATGTGGTCTGAGGGACGCTATGACTACGACCGACTTCCGAGAGAACGCGTGCCTC CCCGGAGCCATCCCAGTGATGGCTACCATAGAGTAGTTAATGTTGTGCCAAAGAGACCACCTCTGCTAGA CAAGAGACCACCTCTGCTAGACAAGAGACCACCTCTGCTAGCCAGACCTGATGAAGGAGGCTACAGTAGA TATTACAGTCATGTTGATTGCCGAGTATGTGACGAGGGCCGCAGTTTTTCTCATGATCGAAGAAGTGGCC CATCTCACAGTGGAGATGAATCTGGCTACAGATGGCTAAGAGATGATCATTCCACAAGCCGGCAGCCTGA CTACAGGGACATGAGAGACGGCTTCAGAAGAAAGAGTTTCTACTCTTCACATTATTCGAGAGATCGGTCT CCCCATAAAAGGGACGCTCCCTTCTTCAGAGAATCCCCTGTCGGCCGGAAGGACTCCCCACACAGCAGAT CCGGCTCCAGTGTCAGCAGCAGAAGCTATTCTCCAGAGCGAAGCAGGACTCACTCCTTCCATCAGTCTCA GCATAGAAAGTCCTCGCGTGTCGGTGCTTCCTACAAACGACAGAATGAAGCAATTCGTGGAAGAGGTAAA GAGAGATCCATCCAGTCAGTGAAAACCTCGAGAGATGCGTCACCCTCAAGTTCCTCAGCAGTTGCTTCAT CCAAGGCGTTAGACAAACCCAGCAGGCTAACTGAGAAGGAACTTGCTGAGGCTGAAAGCAAGTGGGCTAA TGAAACACTAGAGAAGTCAGACGAAAGTAACTTGGCTGAAATGAATGAGTTTGAGGCGGGATCCACGGCA CCCTTATTTATTGACCAGACAGAAGAACCCGAGTCAAACACAGTAGATGGTACAGAACTGTATGAAGACA GCCAGCTCAGCAACCGCTCTAAAGCCATTGCCTCGAAAACCAAAGAGATTGAGCAGGTTTACCGACAAGA CTGCGAGACTTTCGGGATGGTGGTGAAAATGCTGATTGAAAAAGATCCCTCATTAGAAAAGTCTGTCCAG TTTGCACTGAGGCAGAACTTACACGAGATAGGTGAGCGCTGCGTGGAAGAGCTCAAGCGTTTCATTACTG AGTATGACAACTCTGCTCGGGACTTTGGAGACCCCTTTTAGAAGAAGAAGTACCCAGGTCAAAAGGTTTT CTTAGATTGTTTTTTTCTCCTTGGATTGTGTAAATCCTTACTATTTTGTGATGAAGAGAAATACTTTATG ATAGCTAAGCACATTTCTAGTACTAAATAAACATCTACAATAGCTTAAAGTATCTTAATTAGAATGTTTC CCCTATGTGTAGAATATTCTACTCTATCTTCATACATTTCCTCCCTCCAAAAATAATATTCAGTTACTTT AATGTTCAAAAGAAAAAAGCAGCTCTGGAGAGCAGCTCCCTGTATTTAACCCATGTTTCATAGTCTTCCA TGACGTCTATGATCATCTTTTTGCATACCGTGTAAAGGTTGTCTTTTTTATTTTGTTTTGTGTTTTTTGT TTTTTTGTTCTTTTTTTTTCGAGACAGGGTTTCTCTGTGTATCCCTGGCTGTCCTGGAACCCACTCTGTA GACCAGGCTGGCCTCTAACTCAGAAATCCGCCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGGCGTGCA CCACCACTGCTGTATTCAGATGTTGATTTCCAGCTGAATACTCTTTGGTATTGTTATTTGAATTGCTAAG CACCTTCATTGTAGTTTGTAAACTCTCCTCTCCACCACCTTCTGATTGGTAAACACTGAGGGCCTGTAGG TCAGGAAAGGAAAGGCAGGGCAGGGCAGGGCATCACAAAAGGAAGTCTGACTTTCAGGGACTGAAAGATA ACGGGCCATGTGACAAACATGGACCAAAATAAATGGATTAGTTAAGCCATAGGAGCTAGTTGGGAAGAAG CCTAAGATATAAGGCCTTGGCATTCATAAATAATAAAAAGTCTCTGTGTTATTGTTGAGAGTCAGGGGTC GAAACAGTCTAACAGTAGTGATCATTTGGTCTCAGGAGTTTAATTTGTAGTTAAGATCATTCCAGATTGC ATACAATTAGTGGTACCGTTAGCATTTTGAGTTGGTGATGTCAAAATATGCTGTCTTAATGAGTTAGAAC TTTGCTATCATTCAATATTTGCTTTGAGAATAATTCTTATTCAATATACAAAAAAATGCACTTGAAAATC TGTAAGGATCTGTTAGAACAAAATCTTCTCCCAAGTCAGTAAACTTGGTTCATCTCTTCTATGAAGTGCT CTTAAGGCTTCTACCTGCTCTCTCTAGACAATATTATGGACTAACAGGAGAGGTAATCTGAGATGCAAGA TGAATATTAAGTTGAGATGGTCTCAGAAACCCATCTCATGGGTATAATAAAAAAATTAGAATATACAGAG CACTTAAGTTATGTTTTAGGCCCACATTATATCAAAAACGGGACCTGACATGGCACTCATGCTTGGGATT AAGCTATAGATTACTCTGTCCATCTCCGTGATCTCAGTTCTGTGGGCGTAGAGGGGCTCACTGCTCACAA GTGTCCTCAGAACACACTGAGGCCAGCACAGCTGCCAGCAGCTCCTGAGTGAGCCACTGAATCAGTGAAG AGACACTTAGGATCTCTTGCTCACTTCCTGGTCCCTGGTTCACTGAGGCAAAATACTAAATTTGTGACAT TAGGCACCTTAAAAAAAATCACTGCCCAATCAAATTATCTACCTTTTTTTTGGGGGGGGGGGGTCAGGGT CTTACAGTATAGTCCTAGCTAGCCTGAAAATCTCTGTGTAGACTAGGATAGCCTTAAACTCACAGACATC TCCCCACCCCTACCTCTTATATGCTGGCGTTAAGCCCACGGGCCACCCTGCTCAGCATCCTTTTGTTTGT TTTCATTTTCATTTTTTTTCTGTCCTTATTACTTTTTGATTCGAGACAGGGTTTTGCTGTGTGTCCTAGG CTGGCTTCAAACTTTCCTGCTCTGGCCTTTAGCTTTCGGGATTCCTGCCTCAGCTTTCCTGAGCCCCAAT GCTTGCCGTGACCATTGAGTGGCCTCTATGATCCTGTCTGCGGATATCCCTAAACCACATACAGATTATA CTTAAGTAGAAATAGAAGAATGGTTAATTTTAATGAGCCCTAAAATAGATGTGTGAAGATTTGTATTATG CCATTTTGAAAATTTAAAACTAATTGACGACATGTTTTTTGTTTTGTTTGTTTTCTTAAATTCCCCAATG AGAACACTTGTACGTTTTGGCATTGAGTCTTTCCATCCCTTTTCTGCGCACACTCCCCTTCTGAGCGTGT GCTGCGCAATGCCTGTCACTTCAGTAATACCACAAGCGATTTTCCTTCTTCCCTTCCACTAATTCTCAGC TCTTTAAATCAAGTTATTAACAAGCATACGTATAAAATTTGAGGAGCTTCCAAGGCAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_146062 |
| Insert Size | 1146 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC031486, AAH31486 |
| RefSeq Size | 3570 bp |
| RefSeq ORF | 1146 bp |
| Locus ID | 223828 |
| Gene Summary | Component of the HUSH complex, a multiprotein complex that mediates epigenetic repression. The HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3. In the HUSH complex, contributes to the maintenance of the complex at chromatin. Acts as a transcriptional corepressor and regulates the cell cycle, probably via the HUSH complex. The HUSH complex is also involved in the silencing of unintegrated retroviral DNA: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed. May be involved in epithelial differentiation by contributing to epidermal integrity and barrier formation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG205947 | Pphln1 (tGFP-tagged) - Mouse periphilin 1 (Pphln1), transcript variant 1 |
CNY 4,180.00 |
|
| MR205947 | Pphln1 (Myc-DDK-tagged) - Mouse periphilin 1 (Pphln1), transcript variant 1 |
CNY 3,800.00 |
|
| MR205947L3 | Lenti ORF clone of Pphln1 (Myc-DDK-tagged) - Mouse periphilin 1 (Pphln1), transcript variant 1 |
CNY 5,700.00 |
|
| MR205947L4 | Lenti ORF clone of Pphln1 (mGFP-tagged) - Mouse periphilin 1 (Pphln1), transcript variant 1 |
CNY 5,700.00 |
