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Sharpin (NM_025340) Mouse Untagged Clone
CAT＃: MC201259
Sharpin (untagged) - Mouse SHANK-associated RH domain interacting protein (Sharpin), (10ug)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 0610041B22Rik; AW121341; cpdm; RBCKL1; SIPL1 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC016203 sequence for NM_025340
GTGCATTGGTGCCTGGTTCCTTCCGGGAACTGGTATTGTCTTGTGTAGGGCAGCAGCTGTCGTTCGTTCG AACCTTTAAGAAAAGTTCATCCTTGCCTTCTTTTTGTGACCGGTGACCGGTCTGTGACACTGTCTCGGGT CCTGCTGAAAGGTGACTAGCTTGGCCGGACCGTGCCTGGAGATCTGGGACCATTCCTATCGCTTAGCGAG CCATCAGGCTCTGGTAGTGCGCAGCATCGGGTCCTTCTTGTTCTCCAAGCTCTGGGGCATCCGGAACCGA GGCTCACAGTCCGAGTTTCTGGCCGGCGGAAGTACTCGACAGTTCTCTATCACGGAAGCACTCTCGGAGA GTCAGGACTCCGCCGGATCAGAAATGTCGCCGCCCGCCGGCGGTGCGGCAGTGGCTGCAGACCCTGCCTC ACCTGTGGTGCTCCTGGCAGTGCACGCGGCGGTCAGGCCCCTGGGCGCTGGACAGGATGCCGAAGCGCAG CCGCGGAAGCTGCAATTGATAGCGGACCCTGAGCGGCCTGGACGCTTCCGGCTAGGACTGTTAGGCACCG AGCCTGGGGCGGTCAGTTTAGAATGGCCCTTGGAGGCTATTTGTTACACCGTCCGTGGGCCTAACCAACA CGAGCTGCAGCCTCCCCCTGGAGGTCCTGGAACCTTCAGCGTGCACTTCCTCGACCCCGAGGAAGCACAG CAGTGGGCAGCACTGGTGCGAGATGCCACCGCAGAAGGACAAAATGGCAGTGGCAGCCCAGCCCCAGCCC CAGCCCCAGCAATGTGCCCCATTTCTCCACCATGTTCTTCTATGGCTCAAATACCTAAAGCAACCCAACC TGAGGTGGATCTTCCTCAAAGTTCTGGAAACTTCAAAAAAGAAGAGCTGGCTACACGCCTGTCCCAGGCC ATTGCAGGTGGGGATGAGAAAGCAGCGGCCCAAGTGGCAGCAGTCCTGGCGCAGCATCATGTGGCTCTCA ATGTCCAGCTCATGGAGGCCTGGTTCCCACCAGGTCCCATAAGGCTACAAGTCACAGTTGAAGACGCCAC ATCGGTTTTGTCCTCTTCATCGTCTGCCCATGTCTCACTGAAGATCCACCCACATTGCTCCATTGCAGCC CTTCAGGATCAGGTGTTCTCAGAGTTCGGTTTCCCACCAGCTGTGCAGCGCTGGGTCATTGGGCGGTGCC TGTGTATGCCTGAACGAAGCCTTGCTTCCTATGGGGTCTCTCAAGATGGAGACCCTGCTTTTCTTTACTT GCTCTCGGCCCCCAGAGAAGTTTCAGGACAAAGCCTTCAAAACTCCAAGATGGACCGGAAATTAGGCTTG TTTCCTCAGTCACTGGGGCTGCCTCATGACCTCCAGCCGTCCAGTTCCAGTCTCCCAAGTCCCTCTCAGC CTGGCTGGTCTTGCCCTTCCTGCACTTTCATCAATGCCTCAAACCGCCCTGGCTGTGAGATGTGTAGCAC CCAAAGGCCCTGTGCTTGGGATCCTCTTGCTGCAGCTTCCACCTAGAAGCCACCAAAGGTTACAGAGAAT ATGGCCCTCCTGTCACAGGCCCAACCACCACCACTTGAACTTTGCAGGGGACTGATGCTTGTTGGGGACA GAGATGCCAGTGTTAAACAACAGGGGCTACATACAAGCTAGTAATTAAAGACACATACGAGCCAAAAAAA AAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_025340 |
| Insert Size | 1143 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC016203, AAH16203 |
| RefSeq Size | 1688 bp |
| RefSeq ORF | 1143 bp |
| Locus ID | 106025 |
| UniProt ID | Q91WA6 |
| Gene Summary | Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG205930 | Sharpin (tGFP-tagged) - Mouse SHANK-associated RH domain interacting protein (Sharpin) |
CNY 5,256.00 |
|
| MR205930 | Sharpin (Myc-DDK-tagged) - Mouse SHANK-associated RH domain interacting protein (Sharpin) |
CNY 3,656.00 |
|
| MR205930L3 | Lenti ORF clone of Sharpin (Myc-DDK-tagged) - Mouse SHANK-associated RH domain interacting protein (Sharpin) |
CNY 4,750.00 |
|
| MR205930L4 | Lenti ORF clone of Sharpin (mGFP-tagged) - Mouse SHANK-associated RH domain interacting protein (Sharpin) |
CNY 4,750.00 |
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