Ttc8 (NM_198311) Mouse Untagged Clone
CAT#: MC201256
Ttc8 (untagged) - Mouse tetratricopeptide repeat domain 8 (Ttc8), transcript variant 2, (10ug)
CNY 3,840.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 0610012F22Rik; AV001447; BBS8 |
Vector | PCMV6-Kan/Neo |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>BC017523 sequence for NM_198311
CCGGGCCGGGCTCTGGGTACAGGGCCTCTGGGTTCCTTGAGGGTTGTCCCGGACCACCATGGGCTCAGAG ATGGAGCCACTGCTCCGGGCCTGGAGCTATTTTAGACGCAGGAAGTTCCAGCTCTGCGCCGATCTATGCA CGCAGATGCTGGAGAAATCCCCTTATGATCAGGAACCAGCTCCTGATTTGCCAGTGTCTCAGGCGGCTTG GATTTTGAAAGCACGGGCGCTGACAGAGATGGTGTACATAGATGAAATTGATGTGGACCAGGAAGGAATT GCTGAAATGATCCTGGATGAAAATGCTATTGCTCAGGTCCCACGCCCTGGCACATCTTTGAAGCTCCCTG GCACTAACCAAACAGGAGGACCGACTCAAGCTGTCAGGCCAATCACTCAAGCTGGAAGACCCATAACAGG TTTCCTTAGGCCCAGCACACAGAGTGGAAGACCAGGTACAATGGAACAGGCCATCAGAACACCCAGAACT GCCTACACAGCACGACCTATCACTAGCTCATCTGGAAGATTCGTCAGACTGGGGACTGCTTCCATGCTTA CAAGTCCCGATGGACCATTTATAAATTTATCTAGACTGAATTTAACAAAATATTCCCAGAAGCCTAAGTT GGCAAAGGCTCTATTTGAATATATTCTCCACCATGAAAACGATGTTAAGATGGCTTTGGATTTGGCGTCC CTTTCCACGGAGTATTCTCAGTACAAGGACTGGTGGTGGAAGGTACAGATTGGAAAATGTTACTACAGGT TAGGAATGTACCGTGAAGCAGAGAAACAGTTTAAATCAGCCTTGAAGCAGCAGGAAATGGTAGACACATT TCTCTATCTGGCAAAAGTTTATATCATATTGGATCAACCTGTGACTGCTTTAAATCTATTTAAACAAGGC TTAGATAAGTTTCCAGGAGAGGTGACCCTGCTTTGTGGAATTGCCAGGATCTATGAGGAAATGAATAACA GTTCATCTGCAGCAGAGTACTACAAAGAAGTTCTAAAACAAGACAATACTCACGTGGAAGCCATCGCCTG CATTGGAAGCAACCACTTTTATTCTGACCAGCCAGAGGTTGCCCTTCGGTTTTACAGGCGCCTCTTGCAG ATGGGAGTTTATAACTGCCAGCTTTTCAACAACCTGGGCCTGTGCTGCTTCTATGCCCAGCAGTACGATA TGACCCTGACCTCGTTTGAACGTGCCCTTTCCCTGGCTGAAAATGAAGAAGAGGCAGCTGATGTCTGGTA CAACCTGGGGCACATAGCTGTGGGAATCGGAGATACAAACTTGGCCCACCAATGCTTCAGGCTGGCCCTG GTCCACAACAACCACCATGCTGAAGCCTACAACAACCTGGCAGTGCTGGAGATGCGGAAGGGTCACGTTG AACAGGCACGCGCGCTCTTACAAACCGCATCGTCTTTGGCACCCCACATGTATGAGCCACACTTTAATTT CGCAACAGTCTCTGATAAGATTGGAGATCTTCAGAGAAGCTACGTTGCTGCTCAGAAGTCTGAAGTGGCG TTTCCTGAACACGTGGACACACAGCACTTAATTAAGCAGCTGAAGCAGCACTTTGCTATGCTCTGATCAG TGCTTGGGCCACATAAGCCTTCACGGAGGGCCAGCGCACAGGGAAGAAAACGCACTGAGTGCATTTGTAC CAATACTGTGATGCAGACCTCATAAGGCATCCTACAAGACTGCAAACCCGAACCTGTTGTCTGAACTTTG TTGTTTTATAAGGTAACACTAGAAAAACAGGACTTAAGCATTTCCTAGTTTTATCAATTAAAAAATTTAT TGTTAGATACAGACATTTTCAAAGACATATGTCATATATGTTGGGTGCACTTCTGCCATGCTCTCCTCTC CCATTAGTCCCCTTTGTCCCCTAGACACTTTCATGTCACCTGTATGGGCATGAGTTTAGGTATCTGACTA AGATCTAGAAGCCGCAGATTAGAGAAGAAACACAATGTATATAGTTGTTAAACATTTTCATGAACCTCAA TTTTTTTATAAATGTGTATTTTAAAACTTGTTAAGCAAAAAAAAAACTCTTTAATCATTAGCTGTCATTA TAAACTTAGGAGGTCAGAAATCTGCTTTAAAATTTTTATTTTTATGGCTAATAATGTGTGCTTTTATCAC AAATGCTTTAATTAGAACATGAAAATTTGTATTTTAAACTATTTTTATCATAAAGTTTGTATTATATATT ACACTTTAAAATAAATCAGTATGTAAAACAAAAAAAAAAAAAAAAAA |
Restriction Sites | RsrII-NotI |
ACCN | NM_198311 |
Insert Size | 1548 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC017523, AAH17523 |
RefSeq Size | 2287 bp |
RefSeq ORF | 1548 bp |
Locus ID | 76260 |
UniProt ID | Q8VD72 |
Gene Summary | The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains an additional in-frame coding exon compared to variant 1, resulting in a longer isoform (2) with an internal protein segment not found in isoform 1. |
Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG208262 | Ttc8 (tGFP-tagged) - Mouse tetratricopeptide repeat domain 8 (Ttc8), transcript variant 2 |
CNY 4,940.00 |
|
MR208262 | Ttc8 (Myc-DDK-tagged) - Mouse tetratricopeptide repeat domain 8 (Ttc8), transcript variant 2 |
CNY 3,840.00 |
|
MR208262L3 | Lenti ORF clone of Ttc8 (Myc-DDK-tagged) - Mouse tetratricopeptide repeat domain 8 (Ttc8), transcript variant 2 |
CNY 6,370.00 |
|
MR208262L4 | Lenti ORF clone of Ttc8 (mGFP-tagged) - Mouse tetratricopeptide repeat domain 8 (Ttc8), transcript variant 2 |
CNY 6,370.00 |