Apln (NM_013912) Mouse Untagged Clone

CAT#: MC201204

Apln (untagged) - Mouse apelin (Apln), (10ug)



  "NM_013912" in other vectors (4)

CNY 2,000.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 6030430G11Rik; Apel
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC020015 sequence for NM_013912
GCCTGGACCAACGGGTAGTTGAGAGTCGCGGGCAGAGCAGCGTCAGCCACTTAGAGAGTTTTTGCCGCCG ACCCGAAGCCACCAAGGCCAGCTTCGCGGCGCTGCCCCGCGGCGGCAGAGAAGGCTGCACCAGAGCAGAG GCAGCGAGCAGGAGTGGGGCAGGCAGCCAGCGGTGCGGCTGGGGCGCTCACCCTCCCGCGGTCCGGGAGC CACGCGAGCTCCGTGCCCGCACGCGCCAGCCCCGGCTCGCGCCTTTCTTTGCGTCCGGGTGCCCTGCCTC TCCGCCCACTCGCCGGCTCCTCTGGGCTGCCGCGGACCGAGTTGCAGCATGAATCTGAGGCTCTGCGTGC AGGCGCTGCTGCTGCTCTGGCTCTCCTTGACTGCAGTTTGTGGAGTGCCACTGATGTTGCCTCCAGATGG AACAGGACTAGAAGAAGGAAGCATGCGCTACCTGGTGAAGCCCAGAACTTCGAGGACTGGACCAGGAGCC TGGCAGGGAGGCAGGAGGAAATTTCGCAGACAGCGCCCCCGGCTCTCCCATAAGGGCCCCATGCCTTTCT AAAGCAGGATTGAAGGGCTCGCCAAGTGCCCTCCCGGTGCCGGTCTCTCTACTCCACAGATGAATTCTCT TCTCTGGAACCCTCACATCTATTTGGCTTTCATCTTGCACCTGTTCTAGCTGCTGATGGTCCCGGCTCTT CTCACCCACCAAGTTCCTCTAATGGCGTGGTCTCGCCCCTCACTTGGATGATTTCATTCCTCTCACAAGG ATCCCTTTGGCCCATCCTCCTGGCCACTCTTTGGACTGCCCTTGGAGACCCAGCTTCTCCTTGACCTGTT CCATGCGGTAGTCCCTATCTTTCAGCCGTAGCCTTCTCTTGGCTCTTCCCTCTTTTCATTTGCCTTGGGC ACTGGACAAGAACACTCCCTTCTCCAGTGGATTCCAGCACATCCATGCTTTCTTATCAATTGGACCTCTT GCCTGGAAGGGAGCGCCGAGATGTGCAATGAGACTTCGGTTGAGAGGGGTTGGGTGTTGGGGTCCAGATA AGGTGCACTGGGGAAACTGAATGGTGGGCGTGAGGAAGGGGTGTGGCGAAATAGCTGCCACAGCCTCTTC CTTAGCTCTCTCTGGCCCTGTTAAAGGAAGACGCAAAGGAAAGGAGCCCATGAAGTTCTATGTCACCGTG CCTTCTAATGTCTTCGCTCTCTCTGTTCTATTGCAGCTTGTTCAGTCCCCAGGTCAGAAGGGGAGAGTGT GGATCCTGACATGGTTCTATACTCTTCTGGGGGTAATTTTGCCAAAAAAATAATCCCAACCAATCAACCA ACCAACCAAATAACCCACAGAAAGTCAGAGCCCAAAGGACCCTTGAAGATCACTTAGCTCCCTTATGCAA AAGCTTGCACAAATAGGCCCTGGAGAGGGAAGTAACCTGCTTAGAGCCACTGCAGAGCCCCACAGGGCAC CAGGGCCCTCAACTCCCACTTTATGATGCCCTTTTTCCTCTTGATGATGTCCTTTCAAAGCAAATGAAGT GCCTTTTGCTGGGTTTAGTGAAGCGGGTGGCTAGGAAGGAAGAGCAGGGAGAGACAAGCCAGCTGTGAAC TGCTTTGTGTGAGAATGGAGCATCTCCCTCCCAACGTGGCCCGGAATAGTCCCTAAGTGGTGAAATGTAC CCCACACAGGCCTATTCCCAGGCTCATGCCATTTTAATTGGGGTTCTCAGGCACTGAAACTACCATTTAG CCCCTGACACTGGTTGTCATGAACCCCTGTGTTGACTGCGCCCTTGATCTTGGGGCTTTTGGGGGATGGA GAAAGGCAAAGAAAGGAAGGAGAGGTGGAAGAAGGAAGGAGAGATGGGAGGATGTGTAGGTGGGATCTAT CACTCCACCAAGGGGGTCTCATCTCTCACCAGGAGCCTTTTGTAGCCCTTCCCTGAAGGGAGACCCTCAT CTTCTCTGCCTGAGCACATGTTCTTCTCAGAGGCCTCTCAGGAGGGAGAAAAGAGAAGACAGAAGCTCGA TATTCACTTATATATGTGTAGGGTAGGGGGAGTCAGGACCCCCAAGCCCCATAATAACCTGATTGCCTAT TCATCCTTTCCCAAAGCCTTCCTATCTGCTGCTGCTAATGCTGCTGCTGCTGCCTCTGCTGCCGTTTCAA GGCCCACCCTGGGGAGCAGGGCCAGGCATAGCGTCCTCACCTCTTGGGCTAGAAGTGGGAGCTGGCTGGC GCCTCTTCTACTGCTGGTACCCCTACCAGGGACCGGGCCTTCTCCGTCTTTGTCGTTTCTGCACCTCCTG CCTTATCAGCCTGCCATGGCCAGCCCCACAGGGAAAGGGAGGGGGGAATATTGGCTGAGAAAGAGGAGAG ATAGAAGCACAAGAGGGGAGTAAACGGGCCCAGTGGGCTGTCTTATTTAAAGTGGTTATGTGTATTTCTT ATACTAATTTATATAGAGATATTAAGGCCCTTTGAGTTAGAGAAACTGTCCCCTTCCCATAAGTGTGTTC GCTATGTTTGTAAAAATTGTTCCATGTAAATATGTCTTTATAATAAAGAGTTAAAAGTTGACAGTTTGCC CTTACTCTTTGAGGTCATATTCAGGAGGGCATTCTTTTTCTCCTAGGGCCAATCTTGTCCCTGTGCCCAC AGATTGCACGTGTAGGGAGGTGAGTGCTTGTATCCCAAATTGGTTCTAGGTCAACTGGCCTCAAACTGAT TTGCCACAAGCTCACAAAAAGACTCGCTATGCTTAAGGGCCAGTTCACATAAATTTGAGCCCCTTTTAAG TCCTTTGGCATCTGTTCGAATGTCCTCATTTTTCTGGCAGTGTCACTTAAAGGATTTTTTTTCCAGAAGC AATTCATTTAAGGACACGCTGATCAAAGGTGGGATTTGTACTAAACGCTGTTTTGTAGCCCTGGTGGGTG CCTTCTGTTTTATCTGCTTTTGAATACTTTCGGGACTTTTTAGCCAGTTTGCCTTTCTTGACAAATGTTG TCCTCAGCCATAAATACATTTGGTAATGACTTTGTATGTATCGTTTTATTGTTTCACAAAGTGGAGTTGC TTGATGAATGAGATAACCTGGAAAATAAACTTCGAGGAGTTCGATAGGAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_013912
Insert Size 234 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC020015, AAH20015
RefSeq Size 3143 bp
RefSeq ORF 234 bp
Locus ID 30878
UniProt ID Q9R0R4
Gene Summary This gene encodes the neuropeptide Apelin, an endogenous ligand of the G protein-coupled receptor APJ. This gene is highly expressed in the central nervous system and peripheral tissues and the encoded preproprotein undergoes proteolytic processing to generate mature peptides of different sizes that are secreted into the plasma. These peptides play important roles in a broad range of physiological processes such as cardiac contractility, blood pressure, blood vessel growth, appetite and drink behavior, and pituitary hormone secretion. Mice lacking the encoded protein develop progressive heart failure and exhibit a shorter bleeding time, prothrombotic profile and increased bone mass. [provided by RefSeq, Jul 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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