Lypla1 (BC013536) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Pla1a
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC013536 GCACTGTCCGCCAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCG CCCGGAAGGCCACCGCCGCGGTTATTTTCCTTCACGGATTGGGAGATACAGGGCATGGATGGGCAGAAGC CTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAGTCACATTA AATATGAATATGGCTATGCCTTCTTGGTTTGATATCGTTGGACTTTCACCAGATTCCCAGGAAGATGAAT CTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGATAGATCAAGAAGTGAAGAATGGCATTCCTTC TAACAGGATTATTTTGGGAGGATTTTCTCAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACACAG CAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGCTTCGTTTTCACAGGGGCCGA TCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTGACCCTTTAGTTCCCCTAAT GTTTGGTTCTCTTACTGTTGAAAGACTAAAAGCATTGATAAATCCAGCCAATGTAACCTTCAAAATCTAT GAAGGCATGATGCACAGCTCATGTCAGCAGGAAATGATGGATGTCAAGCACTTCATTGATAAGCTCCTAC CTCCAATTGATTGACATCACTAAGAGGCCTTGAGTAGAAGTTCACCAGCATCACAGTAGTAGAGTATAAA CCTCTTCCCATGACTGACCATAGAACTTCTAATGTGTGCAGTGTTAAAATGTTTTGCAAATACATACCAA TGATACAGACTAAATGATGTTACCCTCATGGGAAATATATGATCCTTTTAAGTGTCTATGCATATATTTG TATTATACAATTCAGAATATACTAAGTATTACAGTAGGTAAGTGAAGCAACTAGATTCTTTGTCTCAAAT TCAGCAAAATAAGATAACAAACAGTTTTATTACATCATTTGAAAATTACTAGTATGTTCTGTGAAGATGT GTTCAGGTAAGATGTAAGTGACTTCTTAGTTCTGTGAGTTAGACTATAGTTTTACTCCACAATTGTTCAG TCTCCATGCAGTGTCTGTATTTTTCTGTTTGTATTCACATAGATGATACTATAATAGCTAAGAATCAGAT GGTATTACATTATCCCTAATAATGGGGATAATGCTTTTGAGTGTCAAAAAGTAATACTGCATTCTTCAAT TAATGGCCCTTCTATTGGGAGACCAGGCTTTTTTTCCCTTCTATGTAATTTTTCTCAAGAAAAAACAAGG TTCTTTTTTGATTTTCCGTAGAAAACTAATATTGCCTCTCTGTCATATATTTTATTTTGACATTTTGAGA TACAGACATAGCCGGGCGTGGTGGTGCATGCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCGGATC TCTGTCAATAGCCTGGTCCACAGAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGG AAACAAACAAACAAACAAACCGAAACACAGACATACAGTATTTCCAACCTAGGTAATATGAAAAGAAATC AAACATTACTTAAGTTTATATATTTCAAATAACTGCACCTTAGTCCAACTTTAGACTGTAGAGTTATAGA CTCTCCAGTCCTGGTAATAAATCTGTGGTTTTATTTTTCTGTAAAACACTTAAAGGGGAAGTAATATCTT GGCATATTTAGAAATACTTGTGACTGGCTTCAAAATATAATTTCTTAAATATCTTATAGCCGATTTTAAG TTTACATTTTCTGTACATGTTATGCTTAAAACCAAATACTGTTCAAGATGAGCAATCAGACTTATGTTTT ATTTTTATCTGGGTAACATATTATTTTGAAATTTTCATCTTAAGTAAAGGAGCTTTTGATAATCAGTCTC CTGTACAAATATTAAAATAATAATTTGATTTTAAATCATCAGCATGTTCAAAATACTGGTGAAAATTATT TAATATAAGGACTGGTTAGAACATTATATATATATCCCATATTTATAACTGTTACCACACAGTTGAATAT GTTGCAAAAATTATGGTTATTTCTGAACGGTTGCTTTTCTTGCTGTTTTCCAACCAACCAAGAAGCACCT TTATAAACAGGTGCTAATTGATATGAAGATCATTTTGGTATGTCCTAGATAGAATGAAGCCAAATGTCTG GATTTAAAACAATTTCAAACAATCATTTACTATTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGTT ATTCTATCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATG TCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTTAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC013536
Insert Size	693 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC013536, AAH13536
RefSeq Size	2447 bp
RefSeq ORF	693 bp
Locus ID	18777
Gene Summary	Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has depalmitoylating activity toward KCNMA1. Has low lysophospholipase activity (By similarity).[UniProtKB/Swiss-Prot Function]

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