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Hsf1 (NM_008296) Mouse Untagged Clone
CAT＃: MC200965
Hsf1 (untagged) - Mouse heat shock factor 1 (Hsf1), (10ug)
CNY 2,000.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AA960185; Hsf1alpha; Hsf1beta; HSTF |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC013716 sequence for NM_008296
CGCCGGTCGCAAGATGGCTGCGGCCATGCGAGGCCTTGCTGTGTGTGCGCAGCGGGCGGCGGAGCGGCCC GGACGGCAGGGGCAGCGACGACACTAGCTCAGCCTTCAGCCACTCTTCTAAAAGGCCACCCCAGCCTCTG CCTGCTTTCGTCCGAGATGGATCTGGCCGTGGGCCCCGGTGCAGCGGGGCCCAGCAACGTCCCGGCCTTC CTAACCAAGCTGTGGACCCTCGTGAGCGACCCGGACACAGACGCGCTCATCTGCTGGAGCCCGAGTGGGA ACAGCTTCCACGTGTTTGACCAGGGCCAGTTTGCCAAGGAGGTGCTGCCCAAGTACTTCAAGCACAACAA CATGGCTAGCTTCGTGCGGCAGCTCAACATGTATGGCTTCCGAAAAGTAGTCCACATTGAGCAGGGTGGC CTGGTCAAGCCTGAGAGAGATGACACCGAGTTCCAGCATCCTTGTTTCTTGCGTGGACAGGAACAGCTCC TTGAGAACATCAAGAGGAAAGTGACCAGCGTGTCCACCCTGAAGAGTGAGGACATAAAAATACGCCAGGA CAGTGTCACCCGGCTGTTGACAGATGTGCAGCTGATGAAGGGGAAACAGGAGTGTATGGACTCCAAGCTC CTGGCCATGAAGCACGAGAACGAGGCCCTGTGGCGGGAGGTGGCCAGCCTTCGGCAGAAGCATGCCCAGC AGCAAAAAGTTGTCAACAAGCTCATTCAGTTCCTGATCTCACTGGTGCAGTCGAACCGGATCCTGGGGGT GAAGAGAAAGATCCCTCTGATGTTGAGTGACAGCAACTCAGCACACTCTGTGCCCAAGTATGGTCGACAG TACTCCCTGGAGCATGTCCATGGTCCTGGCCCATACTCAGCTCCATCTCCAGCCTACAGCAGCTCTAGCC TTTACTCCTCTGATGCTGTCACCAGCTCTGGACCCATAATCTCCGATATCACTGAGCTGGCTCCCACCAG CCCTTTGGCCTCCCCAGGCAGGAGCATAGATGAGAGGCCTCTGTCCAGCAGCACTCTGGTCCGTGTCAAG CAAGAGCCCCCCAGCCCACCTCACAGCCCTCGGGTACTGGAGGCGAGCCCTGGGCGCCCATCCTCCATGG ATACCCCTTTGTCCCCAACTGCCTTCATTGACTCCATCCTTCGAGAGAGCGAGCCTACCCCTGCTGCCTC AAACACAGCCCCTATGGACACAACCGGAGCCCAAGCCCCCGCACTCCCGACCCCCTCCACCCCTGAGAAG TGCCTCAGCGTAGCCTGCCTAGACAAGAACGAGCTAAGTGATCACCTGGATGCCATGGACTCCAACCTGG ACAACCTGCAGACCATGCTGACAAGCCACGGCTTCAGTGTGGACACCAGTGCCCTGCTGGACATTCAGGA GCTTCTGTCTCCACAAGAGCCTCCCAGGCCTATTGAGGCAGAGAACAGTAACCCCGACTCAGGAAAGCAG CTGGTGCACTACACGGCTCAGCCTCTGTTCCTGCTGGATCCTGATGCTGTGGACACAGGGAGCAGTGAGC TGCCTGTGCTCTTTGAGCTGGGGGAGAGCTCCTACTTCTCTGAGGGGGATGACTACACGGATGATCCCAC CATCTCTCTTCTGACAGGCACTGAACCCCATAAAGCCAAGGACCCCACTGTCTCCTAGAGCTCTCAGGAG TTGTCAGGCTGGCTTGTGCCTGGCCCCCAACCTATCCCTAGGACATGGCTGGTCCTAGGGAGACAAAACA GTTGGGTAGTCCAGGGGACCCTAGGTCAAGCCACCACAACCCCAGTGGAGCACAGATGGAACTTGGTCCT GGGCAGTACCTTGGATCAGGAGGAAGATCCTGAGGGCTGCATACCTGCTGCCTTTACCCCAGCCCCAGGT CTACTCTCTGGTCACAGCTTCACAGCCACACTTGGACTGACCCTGCAGGTTGTTCATAAAATTGTATTTT GGATTTTTACATGAGAGTCCCCTTTAACCCTTCACAAATAAATATATATATACATACAAAAAAAAAAAAA AA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_008296 |
| Insert Size | 1512 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC013716, AAH13716 |
| RefSeq Size | 2032 bp |
| RefSeq ORF | 1512 bp |
| Locus ID | 15499 |
| UniProt ID | P38532 |
| Gene Summary | Function as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones heat shock proteins (HSPs) that protect cells from cellular insults' damage. In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form. Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes. Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form. Binds to inverted 5'-NGAAN-3' pentamer DNA sequences. Binds to chromatin at heat shock gene promoters. Plays also several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells. Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK)-dependent manner. Plays a role in nuclear export of stress-induced HSP70 mRNA. Plays a role in the regulation of mitotic progression. Plays also a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage-dependent manner. Involved in stress-induced cancer cell proliferation in a IER5-dependent manner.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. It encodes isoform beta which is shorter compared to isoform gammaalpha. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208087 | Hsf1 (tGFP-tagged) - Mouse heat shock factor 1 (Hsf1) |
CNY 4,850.00 |
|
| MR208087 | Hsf1 (Myc-DDK-tagged) - Mouse heat shock factor 1 (Hsf1) |
CNY 3,744.00 |
|
| MR208087L1 | Lenti ORF clone of Hsf1 (Myc-DDK-tagged) - Mouse heat shock factor 1 (Hsf1) |
CNY 6,270.00 |
|
| MR208087L2 | Lenti ORF clone of Hsf1 (mGFP-tagged) - Mouse heat shock factor 1 (Hsf1) |
CNY 6,144.00 |
|
| MR208087L3 | Lenti ORF clone of Hsf1 (Myc-DDK-tagged) - Mouse heat shock factor 1 (Hsf1) |
CNY 6,270.00 |
|
| MR208087L4 | Lenti ORF clone of Hsf1 (mGFP-tagged) - Mouse heat shock factor 1 (Hsf1) |
CNY 6,144.00 |
