Klf4 (BC010301) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	EZF; Gklf; Zie
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC010301 CCCGCGCCCATGGCCGCGCGCACCCGGCACAGTCCCCAGGACTCCGCACCCCGCGCCACCGCCCAGCTCG CAGTTCCGCGCCACCGCGGCCATTCTCACCTGGCGGCGCCGCCCGCCCACCGCCCGGACCACAGCCCCCG CGCCGCCGACAGCCACAGTGGCCGCGACAACGGTGGGGGACACTGCTGAGTCCAAGAGCGTGCAGCCTGG CCATCGGACCTACTTATCTGCCTTGCTGATTGTCTATTTTTATAAGAGTTTACAACTTTTCTAAGAATTT TTGTATACAAAGGAACTTTTTTAAAGACATCGCCGGTTTATATTGAATCCAAAGAAGGATCTCGGGCAAT CTGGGGGTTTTGGTTTGAGGTTTTGTTTCTAAAGTTTTTAATCTTCGTTGACTTTGGGGCTCGGGTACCC CTCTCTTTTCTTCGGACTCCGGAGGACCTTCTGGGCCCCCACATTAATGAGGCAGCCACCTGGCGAGTCT GACATGGCTGTCAGCGACGCTCTGCTCCCGTCCTTCTCCACGTTCGCGTCCGGCCCGGCGGGAAGGGAGA AGACACTGCGTCCAGCAGGTGCCCCGACTAACCGTTGGCGTGAGGAACTCTCTCACATGAAGCGACTTCC CCCACTTCCCGGCCGCCCCTACGACCTGGCGGCGACGGTGGCCACAGACCTGGAGAGTGGCGGAGCTGGT GCAGCTTGCAGCAGTAACAACCCGGCCCTCCTAGCCCGGAGGGAGACCGAGGAGTTCAACGACCTCCTGG ACCTAGACTTTATCCTTTCCAACTCGCTAACCCACCAGGAATCGGTGGCCGCCACCGTGACCACCTCGGC GTCAGCTTCATCCTCGTCTTCCCCAGCGAGCAGCGGCCCTGCCAGCGCGCCCTCCACCTGCAGCTTCAGC TATCCGATCCGGGCCGGGGGTGACCCGGGCGTGGCTGCCAGCAACACAGGTGGAGGGCTCCTCTACAGCC GAGAATCTGCGCCACCTCCCACGGCCCCCTTCAACCTGGCGGACATCAATGACGTGAGCCCCTCGGGCGG CTTCGTGGCTGAGCTCCTGCGGCCGGAGTTGGACCCAGTATACATTCCGCCACAGCAGCCTCAGCCGCCA GGTGGCGGGCTGATGGGCAAGTTTGTGCTGAAGGCGTCTCTGACCACCCCTGGCAGCGAGTACAGCAGCC CTTCGGTCATCAGTGTTAGCAAAGGAAGCCCAGACGGCAGCCACCCCGTGGTAGTGGCGCCCTACAGCGG TGGCCCGCCGCGCATGTGCCCCAAGATTAAGCAAGAGGCGGTCCCGTCCTGCACGGTCAGCCGGTCCCTA GAGGCCCATTTGAGCGCTGGACCCCAGCTCAGCAACGGCCACCGGCCCAACACACACGACTTCCCCCTGG GGCGGCAGCTCCCCACCAGGACTACCCCTACACTGAGTCCCGAGGAACTGCTGAACAGCAGGGACTGTCA CCCTGGCCTGCCTCTTCCCCCAGGATTCCATCCCCATCCGGGGCCCAACTACCCTCCTTTCCTGCCAGAC CAGATGCAGTCACAAGTCCCCTCTCTCCATTATCAAGAGCTCATGCCACCGGGTTCCTGCCTGCCAGAGG AGCCCAAGCCAAAGAGGGGAAGAAGGTCGTGGCCCCGGAAAAGAACAGCCACCCACACTTGTGACTATGC AGGCTGTGGCAAAACCTATACCAAGAGTTCTCATCTCAAGGCACACCTGCGAACTCACACAGGCGAGAAA CCTTACCACTGTGACTGGGACGGCTGTGGGTGGAAATTCGCCCGCTCGGATGAACTGACCAGGCACTACC GCAAACACACAGGGCACCGGCCCTTTCAGTGCCAGAAGTGCGACAGGGCCTTTTCCAGGTCGGACCACCT TGCCTTACACATGAAGAGGCACTTTTAAATCCCACGTAGTGGATGTGACCCACACTGCCAGGAGAGAGAG TTCAGTATTTTTTTTTCTAACCTTTCACACTGTCTTCCCACGAGGGGAGGAGCCCAGCTGGCAAGCGCTA CAATCATGGTCAAGTTCCCAGCAAGTCAGCTTGTGAATGGATAATCAGGAGAAAGGAAGAGTCCAAGAGA CAAAACAGAAATACTAAAAACAAACAAACAAAAAAACAAACAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC010301
Insert Size	1425 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC010301, AAH10301
RefSeq Size	2156 bp
RefSeq ORF	1425 bp
Locus ID	16600
Gene Summary	Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription (By similarity).[UniProtKB/Swiss-Prot Function]

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