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U2af2 (NM_133671) Mouse Untagged Clone
CAT＃: MC200595
U2af2 (untagged) - Mouse U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2 (U2af2), transcript variant 2, (10ug)
CNY 2,400.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 65kDa |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC200595 representing NM_133671.
Blue=ORF Red=Cloning site Green=Tag(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCG CGCCAGATCTCAAGCTTAACTAGCTAGCGGACCGAC ATGGACTTCTTCAACGCCCAGATGCGCTTGGGAGGGTTGACTCAGGCCCCTGGCAACCCAGTCTTGGCT GTGCAGATAAATCAAGACAAGAATTTTGCCTTTTTGGAGTTCCGGTCAGTGGATGAGACGACCCAGGCC ATGGCATTTGATGGCATCATCTTCCAGGGCCAGTCATTGAAGATTCGAAGGCCTCATGACTATCAGCCA TTGCCTGGCATGTCAGAGAACCCCTCTGTTTATGTGCCTGGAGTTGTATCCACGGTAGTCCCAGATTCT GCCCACAAGCTGTTCATCGGGGGTTTGCCCAATTACCTAAATGATGACCAGGTAAAAGAGCTGCTGACA TCCTTTGGGCCTCTCAAGGCCTTCAACTTGGTTAAGGATAGTGCCACAGGGCTCTCCAAGGGCTATGCC TTCTGTGAGTACGTGGACATCAACGTCACAGATCAGGCCATTGCGGGGCTGAATGGGATGCAGCTAGGG GACAAGAAGCTGCTTGTCCAGAGGGCGAGTGTGGGAGCCAAGAATGCCACGCTGAGCACCATCAATCAG ACACCTGTGACCCTTCAAGTGCCCGGCCTGATGAGCTCTCAGGTGCAGATGGGCGGTCACCCAACTGAG GTCCTGTGCCTCATGAACATGGTGCTGCCTGAGGAGCTGCTGGACGATGAGGAGTATGAGGAGATTGTA GAGGACGTACGAGACGAGTGCAGCAAGTATGGGTTGGTCAAATCCATTGAAATTCCCCGCCCCGTGGAC GGCGTCGAGGTGCCTGGCTGTGGAAAGATCTTCGTGGAGTTCACCTCTGTGTTTGACTGCCAGAAAGCC ATGCAGGGTCTAACCGGTCGCAAGTTCGCCAACAGAGTGGTGGTCACAAAATACTGTGACCCTGATTCT TACCACCGTCGGGACTTCTGGTAG ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAG GATGACGACGATAAGGTTTAAACGGCCGGCCGCGGT |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_133671 |
| Insert Size | 921 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC007487 |
| RefSeq Size | 2190 bp |
| RefSeq ORF | 1416 bp |
| Locus ID | 22185 |
| MW | 33.8 kDa |
| Gene Summary | Plays a role in pre-mRNA splicing and 3'-end processing. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Positively regulates pre-mRNA 3'-end processing by recruiting the CFIm complex to cleavage and polyadenylation signals.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG204312 | U2af2 (tGFP-tagged) - Mouse U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2 (U2af2) |
CNY 2,850.00 |
|
| MR204312 | U2af2 (Myc-DDK-tagged) - Mouse U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2 (U2af2), transcript variant 2 |
CNY 2,400.00 |
|
| MR204312L3 | Lenti ORF clone of U2af2 (Myc-DDK-tagged) - Mouse U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2 (U2af2), transcript variant 2 |
CNY 4,800.00 |
|
| MR204312L4 | Lenti ORF clone of U2af2 (mGFP-tagged) - Mouse U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2 (U2af2), transcript variant 2 |
CNY 4,800.00 |
