Cd74 (BC003476) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CLIP, DHLAG, HLADG, Ia-GAMMA
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC003476 GCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCGCCTGTGGGAAAAA CTAGAGGCTAGAGCCATGGATGACCAACGCGACCTCATCTCTAACCATGAACAGTTGCCCATACTGGGCA ACCGCCCTAGAGAGCCAGAAAGGTGCAGCCGTGGAGCTCTGTACACCGGTGTCTCTGTCCTGGTGGCTCT GCTCTTGGCTGGGCAGGCCACCACTGCTTACTTCCTGTACCAGCAACAGGGCCGCCTAGATAAGCTGACC ATCACCTCCCAGAACCTGCAACTGGAGAGCCTTCGCATGAAGCTTCCGAAATCTGCCAAACCTGTGAGCC AGATGCGGATGGCTACTCCCTTGCTGATGCGTCCAATGTCCATGGATAACATGCTCCTTGGGCCTGTGAA GAACGTTACCAAGTACGGCAACATGACCCAGGACCATGTGATGCATCTGCTCACGAGGTCTGGACCCCTG GAGTACCCGCAGCTGAAGGGGACCTTCCCAGAGAATCTGAAGCATCTTAAGAACTCCATGGATGGCGTGA ACTGGAAGATCTTCGAGAGCTGGATGAAGCAGTGGCTCTTGTTTGAGATGAGCAAGAACTCCCTGGAGGA GAAGAAGCCCACCGAGGCTCCACCTAAAGTACTGACCAAGTGCCAGGAAGAAGTCAGCCACATCCCTGCC GTCTACCCGGGTGCGTTCCGTCCCAAGTGCGACGAGAACGGTAACTATTTGCCACTCCAGTGCCACGGGA GCACTGGCTACTGCTGGTGTGTGTTCCCCAACGGCACTGAGGTTCCTCACACCAAGAGCCGCGGGCGCCA TAACTGCAGTGAGCCACTGGACATGGAAGACCTATCTTCTGGCCTGGGAGTGACCAGGCAGGAACTGGGT CAAGTCACCCTGTGAAGACAGAGGCCAGCTCTGCACAGCAGCAGCGCCCCCTGCTCTCCTGTGCCTCAGC CCTTCTTATGTTCCCTGATGTCATACCCCACTTCCCGTCTCCCTGCACCCTGGGGCTTGAGACTGGTGTC TGTTTCATCGTCCCAGGACACGGCAAATGAAGTCAGAACAGAAGGAGGACGCTGGAGGGCCTTGCTGGCT ACCGCTATAAAGGCCCGCAGACAGGGACAAGGGATGCCCTACCCTTAACCTAGGCTGGACACATTTGCTG CCTTCTCCTCAAGGAAGAAGAACCCAAGCCCCTCCTCCCAGTAACCCCTCCTCACATCCTGCCACCCCCC CTCAAGCCCCACCCCCTTTCAGGTTCCTTGCTCAGCCAAGCTTGTCAGCAGCCTGTAGGATCATGGTTCA AGTGACAATAAAGGAAGAAAGTAGAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC003476
Insert Size	840 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC003476, AAH03476
RefSeq Size	1375 bp
RefSeq ORF	840 bp
Locus ID	16149
Gene Summary	Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place. Enhance also the stimulation of T-cell responses through interaction with CD44.[UniProtKB/Swiss-Prot Function]

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