Gas8 (BC003779) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Gas11
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC003779 GATTAGTGGAAGTGCTGGACTGCTGGCACCATGGCACCCAAAAAGAAAGGGAAGAAGGGGAAAGCCAAAG GGACCGCGATTGTTGATGGCGTCGCACCAGAGGACATGACCAAGGAGCAGGTGGAGGAACATGTCGCCCG CATCCGGGAGGAGCTGGACAGGGAGAGAGAGGAGCGTAACTACTTCCAGCTGGAGCGCGACAAGATCCAC ACCTTCTGGGAGATCACGCGGAGGCAGCTGGAGGAGAAGAAGGCTGAGCTGCGCAACAAAGACCGGGAGA TGGAGGAGGCCGAGGAGAGGCACCAGGTGGAGATCAAGGTATACAAGCAGAAGGTGAAGCACCTGCTGTA CGAGCACCAGAACAACTTGGCTGAGGTGAAGGCTGAGGGCACCGTGGTCATGAAGCTGGCCCAGAAGGAG CACCGCACCCAGGAGGGCGCTCTGCGCAAGGACATGCGGGTGCTGAAGGTGGAGCTCAAGGAGCAGGAGC TGGCTAACGAGGTGGTCATAAAGAACCTGTGCCTGAAACAAGCCGAGGAGATCACCAAGATGCGAAATGA CTTTGAAAGGCAAGTTCGAGAAATTGAGGCCAAGTATGATAAGAAGATGAAGATGCTTAGAGACGAACTT GACCTGCGGAGGAAGACTGAGATCCATGAGGTAGAGGAGAGGAAAAACGGCCAGATCAGCACTCTGATGC AGCGCCACGAGGAGGCCTTCACGGACATCAAGAACTACTACAACGACATCACTCTCAACAACCTGGCCCT CATCAACTCCCTCAAGGAGCAGATGGAGGACATGCGTAAGAAGGAGGAACACATGGAGAGGGAGATGGCG GAGGTGACTCTGCAGAACCGGCGGCTGGCCGACCCGCTGCAGAAAGCCAAGGACGAGATGAACGAGATGC AGAAGAGACTGGGGAACCACGAGAGGGACAAGCAGATTCTGGTCTGTACAAAGGCACGTTTGAAGGTGGC TGAGAGGGAGCTAAAGGACCTGAAATGGGAACATGAGGTCCTGGAGCAGAGGTTCATCAAGGTGCAGCAG GAGAGGGAAGAGCTCTATCGGAAGTTTGCAGACGCCATCCAGGAGGTGCAGCAGAAGACGGGCTTCAAGA ACCTGCTGCTGGAGCGGAAGCTGCAGGCCCTGAACGCTGCTGTGGAGAAGAGAGAGGTTCAGTTCAATGA GGTGCTGGCCGCCTCCAACCTGGACCCCACAGCATTAACGCTTGTGTCCCGCAAACTTGAGGATGTTCTA GAATCGAAGAATACCACCATCAAGGACTTACAGTATGAGCTGGCCCGGGTCTGCAAGGCCCACAATGACC TGCTGCGTACATATGAGGCGAAGTTACTGGCCTTCGGGATCCCCCTGGACAATGTGGGGTTCAAGCCCCT GGAGACAGCAGTGATTGGACAGACACTGGGACAGGGCCCTGCAGGACTTGTGGGTGCCCCTACATAGCCA TCTTCTCAGCCTTCAACCTTTACAGCCTTCCCCTCTGTCCAAGTAATACCTCTATGCCCAACACAGGCAA GGGTTTGTTTTCTAGACTTACCCGTCAGCAATGAAAGTTTCCCACATTTACTTTAAAAAAAAAAAAAAAA AAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC003779
Insert Size	1437 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC003779, AAH03779
RefSeq Size	1620 bp
RefSeq ORF	1437 bp
Locus ID	104346
Gene Summary	Component of the nexin-dynein regulatory complex (N-DRC), a key regulator of ciliary/flagellar motility which maintains the alignment and integrity of the distal axoneme and regulates microtubule sliding in motile axonemes. Plays an important role in the assembly of the N-DRC linker (By similarity). Plays dual roles at both the primary (or non-motile) cilia to regulate hedgehog signaling and in motile cilia to coordinate cilia movement. Required for proper motile cilia functioning. Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by faciltating the trafficking of SMO into the cilium and the stimulation of SMO activity in a GRK2-dependent manner (PubMed:17366626, PubMed:21659505, PubMed:27472056). May play a role in the spermatozoa motility (PubMed:11751847).[UniProtKB/Swiss-Prot Function]

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