Rac1 (BC003828) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AL023026; D5Ertd559e
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC003828 CCCACGCGTCCGGCTTTGGGTGGTGGCTGCTGCTGCGCTTCGCCTTCCCGTGCGCTGAGGGCCCGGCGGC GTGGACAGCAGTCCCGGCACTCGGCGCCCCGCCGCCCGCAGCCTCGCCCAGCGCTTCCCGAGCCCGCCGC TTCGCGCCTCAGCCACCGGCCGCCGCCGCCGCCGCCGCGGCCCACCGAGCGGCCCAGATGCAGGCCATCA AGTGTGTGGTGGTGGGAGACGGAGCTGTTGGTAAAACCTGCCTGCTCATCAGTTACACGACCAATGCATT TCCTGGAGAGTACATCCCCACCGTCTTTGACAACTATTCTGCCAATGTTATGGTAGATGGAAAACCAGTG AATCTGGGCCTATGGGACACAGCTGGACAAGAAGATTATGACAGATTGCGTCCCCTCTCCTACCCGCAGA CAGACGTGTTCTTAATTTGCTTTTCCCTTGTGAGTCCTGCATCATTTGAAAATGTCCGTGCAAAGTGGTA TCCTGAAGTGCGACACCACTGTCCCAATACTCCTATCATCCTCGTGGGGACGAAGCTTGATCTTAGGGAT GATAAGGACACCATTGAGAAGCTGAAGGAGAAGAAGCTGACTCCCATCACCTACCCGCAGGGGCTGGCCA TGGCGAAAGAGATCGGTGCTGTCAAATACCTGGAGTGCTCAGCTCTCACACAGCGAGGACTCAAGACAGT GTTTGACGAAGCTATCCGAGCGGTTCTCTGTCCCCCTCCTGTCAAGAAGAGGAAGAGAAAATGCCTGCTG TTGTAAATGTCGGAGCCCCTCGTTCTCGGTCCTGCCTGGAACCTTTGTACGCTTTGCTCAAAAATCAGCG AGCCTTCGCATTTGATGCCAAGTTTTTGTTACAGATTAATTTTTCCATAAAACCATTTTGAACCAATGAA CCAGTCAATAATTTTAAGGTTCTGTTTTAAATGTAAGAATTCCAACTTACAGTCTATTAAAATTCAGCCC TAAAATGACAAAGCCTTCTTAAAGCCTTATTTTTAAAATCCCCCATTCTTGCTCAGATTAAAAATTGCCA AAATACCTTCTGAACTAAGTTGCGTTGTGCTGAGAACACCTAAGCACTAAACTCTCTTGAGAGACTTCTG TTGCTAAGAAGACCGCAGCTTCTGGAGCCAGGGGTGCAGACCCTTCCAGAGTTCCCAGACCGTGTGACAC AGCACAGCCTCCTTAATGACACGTTGCCATGTAACGCACCTGTAACTTATCAGCCCATGTTCATTACGTA ACTTTGTACTGTACGTCACAGTGATGAGTGTGACAGCTCAGCTCTTTGATTTCATAGTGAGTTTTCTAAA AGACCAGCCGACTAGCTTTTGCAGACTTTGAACAGAACTCTGGTTCCTGTGTTGCCTCTAATGAAGTATT CTGTTCCTAGTTGTGGGTGTGCTGGGTGGAGTGTGAAACACGACATGATCAAAGGAGACAGACAGTATTT TGACTAATATGAAGTAGAGATTACTTTACACTACATTGTACATGGAGTAATTCAACTGAATAAAAGTGTC ACGGGTAAAGCTTTTTAACGGTTAATTTCTGTCAAACAGTAGGTGACAAATGGCCGATCTTATCAGTGTC TCTTGAGCATCCCTTCGCGCCCCCCACCCTCCACTGCCGTCCCTCCCCAGATGGGGCGTTGAGTCCATAT TTAAACTGGCCACCCTCACAGTTCTAACTTAGCAAGTGCTTTTCTTTAGAACCCCTTCTTAACGAGCAAT ATGTCTGACCTGTACTATAAGATCTTTCTGATAACGCAGAGATTTTTTGTTTTTTTTTGGTAGCTCAGTA GAAGTGCGTCCTGTTTTCGCCTTACTTTACTCAGAGCTAGTTAGTGCTTCCTTAGTTTCCTAGCAACTAG GTGTGCAAATCATGTGTTGCAGCTTTACGGTTTTTAAACTATTTTAGATATTCTTAAACTATGAACCTTC TAAACAGTACCAACACTGTCATGTGACTAATGCTGCCTCTAGACCTCGCCCACGTGGACAGACGCTTCCT GTCATGGCTCTGCCTAGAGATGTTCCTTGGGGTCTGTGAGGTTCTGTCAACTGCTAGTGCTAACGCTGTC CTGTACAACCTAACTCACTGGCAAGAATGCAGTGTTGGGCCTTTCAACCACTAGAACAAACTTTTTTCAA TTGACAGTTGCAGAATTGTGGAGTGTTTTTACATTGATCTTTTGCTAATGCAGTTAGCAGTATGTTTTGC ATGTATGAATTAATAAATCCTTGAATCATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC003828
Insert Size	579 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC003828, AAH03828
RefSeq Size	2319 bp
RefSeq ORF	579 bp
Locus ID	19353
Gene Summary	Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states (PubMed:24352656). In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization, neurons adhesion, migration and differentiation, and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. In neurons, is involved in dendritic spine formation and synaptic plasticity (PubMed:24352656, PubMed:26969129). In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3.[UniProtKB/Swiss-Prot Function]

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