Eef1a1 (BC004067) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC004067 CGGACGCGTGGGTCAGAACGCAGGTGTTGTGAAAACCACCGCTAATTCAAAGCAAAAATGGGAAAGGAAA AGACTCACATCAACATCGTCGTAATCGGACACGTAGATTCCGGCAAGTCCACCACAACCGGCCACCTGAT CTACAAATGTGGTGGAATCGACAAGCGAACCATCGAAAAGTTTGAGAAAGAGGCTGCTGAGATGGGAAAG GGCTCCTTCAAGTACGCCTGGGTCTTAGACAAACTGAAAGCTGAGCGTGAGCGTGGTATCACTATTGACA TCTCCCTGTGGAAATTCGAGACCAGCAAATACTATGTGACCATCATTGATGCCCCAGGACACAGAGACTT CATCAAAAACATGATTACAGGCACATCCCAGGCTGACTGTGCTGTCCTGATTGTTGCTGCTGGTGTTGGT GAATTTGAAGCTGGTATCTCCAAGAACGGGCAGACCCGCGAGCATGCTCTTCTGGCTTACACCCTGGGTG TGAAACAGCTGATTGTTGGTGTCAACAAAATGGATTCCACCGAGCCACCATACAGTCAGAAGAGATACGA GGAAATCGTTAAGGAAGTCAGCACCTACATTAAGAAAATTGGCTACAACCCTGACACAGTAGCATTTGTG CCAATTTCTGGTTGGAATGGTGACAACATGCTGGAGCCAAGTGCTAATATGCCTTGGTTCAAGGGATGGA AAGTCACCCGCAAAGATGGCAGTGCCAGTGGCACCACGCTGCTGGAAGCTTTGGATTGTATCCTACCACC AACTCGTCCAACTGACAAGCCTCTGCGACTGCCCCTCCAGGATGTCTATAAAATTGGAGGCATTGGCACT GTCCCTGTGGGCCGAGTGGAGACTGGTGTTCTCAAACCTGGCATGGTGGTTACCTTTGCTCCAGTCAATG TAACAACTGAAGTCAAGTCTGTTGAAATGCACCATGAAGCTTTGAGTGAAGCTCTTCCTGGGGACAATGT GGGCTTCAATGTAAAGAACGTGTCGGTCAAAGATGTTAGACGAGGCAATGTTGCTGGTGACAGCAAAAAC GACCCACCAATGGAAGCAGCTGGCTTCACTGCTCAGGTGATTATCCTGAACCATCCAGGCCAAATCAGTG CTGGCTACGCTCCTGTTCTGGATTGTCACACAGCCCACATAGCATGCAAGTTTGCTGAGCTTAAAGAAAA GATCGATCGTCGTTCTGGTAAGAAGCTGGAAGATGGCCCCAAGTTCCTGAAGTCTGGCGATGCTGCCATT GTTGATATGGTCCCTGGCAAGCCCATGTGTGTTGAGAGCTTCTCTGACTACCCTCCACTTGGTCGCTTTG CTGTTCGTGACATGAGGCAGACAGTTGCTGTGGGTGTCATCAAAGCTGTGGACAAGAAGGCTGCTGGAGC TGGCAAAGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAAATGAATATTACCCCTAACACCTGCCACC CCAGTCTTAATCAGTGGTGGAAGAACGGTCTCAGAACTGTTTGTCTCAATTGGCCATTTAAGTTTAATAG TAAAAGACTGGTTAATGATAACAATGCATCGTAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC004067
Insert Size	1389 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC004067, AAH04067
RefSeq Size	1588 bp
RefSeq ORF	1389 bp
Locus ID	13627
Gene Summary	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.[UniProtKB/Swiss-Prot Function]

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