Hspa2 (BC004714) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	HSP70.2, MGC58299, MGC7795, HSP70A2, 70kDa
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC004714 GAGGTCTCCGGGAGACGCTAGTCGCGCTCGTGGAGAGTTGTGAGAAGCGACCGTTGGCTCAGGTCCCGGC TTGCGCTTGCCTCTCCTGACGCTTTCGTCCTAACGTTGCTTTGCCTGTTTTTTCAGTCAGGATGTCTGCC CGCGGCCCGGCTATCGGCATCGACCTGGGCACCACTTACTCGTGCGTGGGGGTATTCCAACATGGCAAGG TGGAGATCATCGCCAACGACCAGGGTAACCGCACCACCCCCAGCTACGTGGCCTTCACTGACACCGAGCG TCTCATCGGCGACGCCGCCAAGAACCAAGTGGCCATGAACCCCACAAACACCATCTTCGACGCCAAGCGG CTGATCGGACGGAAGTTCGAGGACGCCACAGTGCAGTCCGATATGAAGCACTGGCCGTTCCGAGTGGTGA GCGAAGGTGGGAAGCCCAAAGTGCAGGTGGAATATAAAGGGGAGATGAAGACCTTCTTCCCTGAGGAGAT TTCTTCCATGGTCCTCACTAAGATGAAGGAGATCGCCGAAGCCTACCTGGGGGGCAAGGTGCAGAGCGCA GTCATCACTGTTCCTGCCTATTTCAACGACTCGCAGCGCCAGGCCACCAAGGATGCGGGCACCATCACCG GCCTCAACGTGTTGCGCATCATCAATGAGCCCACAGCAGCGGCCATCGCCTATGGCCTGGATAAGAAGGG CTGTGCGGGCGGCGAGAAGAACGTGCTCATCTTTGACCTGGGCGGGGGCACCTTCGATGTGTCCATCCTG ACCATCGAGGATGGCATCTTTGAGGTGAAGTCCACGGCCGGCGATACCCACCTGGGTGGCGAAGACTTCG ACAACCGTATGGTCAGCCACCTGGCGGAGGAGTTCAAGCGCAAACACAAGAAGGACATTGGGCCCAACAA GCGCGCTGTGCGCCGGCTGCGCACCGCCTGCGAGCGCGCTAAGCGCACCCTGAGCTCGTCCACGCAGGCC AGCATAGAGATCGACTCGCTCTACGAGGGCGTGGATTTCTACACGTCCATCACCCGCGCCCGCTTCGAGG AGCTCAACGCCGATCTCTTCCGAGGGACCCTGGAGCCGGTGGAAAAGGCGCTGCGCGATGCCAAGCTAGA CAAGGGCCAGATCCAGGAGATAGTGCTGGTGGGCGGCTCAACCCGCATCCCTAAGATCCAGAAGCTCCTG CAAGATTTCTTCAACGGCAAGGAGCTGAACAAGAGCATTAATCCCGACGAGGCGGTGGCCTACGGCGCCG CTGTGCAGGCGGCTATCCTCATCGGCGACAAGTCGGAAAATGTGCAGGATCTGCTGTTACTCGACGTGAC TCCATTGTCGCTCGGCATCGAAACAGCTGGCGGTGTCATGACCCCACTCATCAAGAGAAACACCACGATC CCCACCAAGCAGACGCAGACCTTCACTACCTACTCAGACAACCAGAGCAGCGTGCTGGTGCAAGTGTACG AGGGCGAACGGGCCATGACCAAGGACAATAACCTCTTGGGCAAGTTCGACCTGACTGGGATCCCCCCAGC ACCCCGTGGGGTCCCCCAGATCGAGGTCACCTTTGACATCGATGCCAACGGCATCCTTAACGTCACTGCT GCGGACAAGAGCACCGGTAAAGAAAATAAAATCACCATAACCAACGACAAGGGTCGGCTGAGCAAAGACG ACATTGACCGGATGGTGCAGGAGGCGGAGCGGTACAAATCGGAAGATGAAGCAAATCGCGATCGCGTGGC AGCCAAAAATGCGGTCGAGTCCTATACCTACAACATCAAGCAGACCGTGGAAGACGAGAAACTGAGGGGC AAGATTAGCGAGCAGGACAAAAACAAGATCCTCGACAAGTGTCAGGAGGTGATCAACTGGCTTGACCGAA ACCAGATGGCAGAGAAAGATGAGTACGAACACAAGCAGAAAGAGCTTGAGAGAGTGTGCAACCCCATCAT CAGCAAACTTTACCAAGGCGGTCCAGGCGGCGGCGGCTCCTCTGGAGGGCCCACCATCGAGGAAGTGGAC TAGGCTTGCGCTGGAGTCCGCGTAAACCTCTTTTCCTTTCTTTTCTTTTTTCTTTTTTTTCCTTTTCTCC CTTTTGTTTTGGTTTCTTTTAAATGTCCTTGTGCCAAGTAAGATCTATTATTGGAAGTCTTTAGCCTGGT GCATACATATGAAAGGAAAGGTGCAACAACTTAGTTTAGTTATAAAAGGTTAGTTCTAAAAGTTTGATTT GGGGGAAAAATGAGGTTTCTCTTTAATGCATTTAGAGCGATTGCTGATTTAAGCTCTTTTTGTTGTTGTT AAGCTTACGTATGTACATGGAGATTTGCTTGAAAGGAGAACCCTGATGCTCGCACACCTGACCTGTGGAA GCTTGGTTACACTAAGACACAGGAAAGCTTGAATTGTTTATTTTTATGTACTATGAGATGAAAGTAAGCA CTGCAGTGATATTAAAGACAGGTATATTCTGTAAACACATAAATGCACATTTAAAGTAAAGCTGAAATTG ATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC004714
Insert Size	1902 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC004714, AAH04714
RefSeq Size	2568 bp
RefSeq ORF	1902 bp
Locus ID	15512
Gene Summary	Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release (By similarity). Plays a role in spermatogenesis (PubMed:24557841). In association with SHCBP1L may participate in the maintenance of spindle integrity during meiosis in male germ cells (PubMed:24557841).[UniProtKB/Swiss-Prot Function]

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