Cops6 (BC004664) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Sgn3, VIP/MOV34
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC004664 CGCGGGGAAAATGGCGGCGGCAGCTGCGGCGGGGGCGAATGGGAGCGGAGGCAGCAGCGGCATGGAAGTG GATGCAGCAGTCCCCAGCGTGATGGCCTCCGGAGTGACTGGGAGTGTTTCCGTCGCTCTTCATCCCCTTG TCATCCTTAACATCTCAGACCATTGGATCCGCATGCGCTCCCAGGAGGGGCGGCCTATGCAGGTGATTGG GGCTCTGATCGGGAAGCAGGAGGGGCGAAATATCGAAGTGATGAACTCCTTTGAGCTGCTGTCCCACACC GTGGAAGAGAAGATTATCATTGACAAAGAATATTATTACACCAAGGAGGAGCAGTTTAAACAGGTTTTCA AGGAGCTGGAGTTTCTGGGTTGGTATACCACAGGGGGGCCACCTGACCCCTCAGACATCCACGTCCATAA GCAGGTGTGTGAGATAATTGAGAGTCCGCTCTTTCTGAAGTTGAACCCTATGACCAAGCACACAGATCTT CCTGTCAGCGTTTTTGAGTCTGTCATCGATATAATCAATGGAGAGGCCACAATGCTGTTTGCTGAGCTCA CTTACACTCTGGCCACTGAGGAAGCTGAACGGATCGGTGTAGACCACGTGGCCCGGATGACAGCAACAGG CAGTGGGGAGAACTCCACTGTGGCTGAACACCTGATAGCTCAGCATAGTGCCATCAAGATGCTGCACAGC CGTGTGAAGCTCATTTTAGAATATGTCAAGGCCTCTGAAGCAGGAGAGGTTCCCTTCAACCATGAGATCC TGCGGGAGGCCTATGCCCTATGTCACTGTCTTCCAGTTCTCAGCACTGACAAGTTCAAGACAGACTTTTA TGATCAATGCAATGACGTGGGGCTCATGGCCTACCTCGGCACCATCACCAAAACGTGCAACACAATGAAC CAGTTTGTGAACAAGTTCAACGTCCTCTACGACCGACAAGGCATTGGCCGGCGAATGCGGGGACTGTTTT TCTGATGATGGTTCTGGAAGGGATGGTGTGTGGGGCTCAGACAGCTGTTCCATGGACCTGAGTACCACAT TCCCTTTAGAGAAACTCATTAATAAAAGAGCAGCCCCTTAGCAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC004664
Insert Size	975 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC004664, AAH04664
RefSeq Size	1118 bp
RefSeq ORF	975 bp
Locus ID	26893
Gene Summary	Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Has some glucocorticoid receptor-responsive activity (By similarity). Stabilizes COP1 through reducing COP1 auto-ubiquitination and decelerating COP1 turnover rate, hence regulates the ubiquitination of COP1 targets, including SFN (By similarity).[UniProtKB/Swiss-Prot Function]

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