Pacsin3 (NM_030880) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4921507A02Rik; 6330413E15Rik; AW413130
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC003884 sequence for NM_030880 CTGAGCGTGGCCAGATCCCCCTAACCCACCGAGGGCCCCTGAGAGGACACGACAGGCTCATAGTGGCAGC TTGGAGACTTGGACACGGGTCCTGGAAGTGATCTGGAGACCCCAGCACCACATCTGCCACCATTCCGTGC TGCAGGGATACCATGGCTCCAGAGGAGGACGCCGGAGGGGAGGTCCTAGGGGGCAGTTTCTGGGAGGCTG GCAACTACAGACGGACGGTGCAGAGGGTTGAGGATGGGCACAGGCTGTGTGGGGACCTGGTTAGCTGCTT CCAAGAACGTGCCCGCATTGAGAAGGCCTACGCCCAGCAGCTGGCTGACTGGGCCCGCAAGTGGAGGGGT GCTGTGGAGAAGGGCCCACAGTACGGCACCCTGGAGAAGGCCTGGCATGCCTTCTTCACTGCGGCTGAGA GGCTGAGCGAGCTGCACTTGGAGGTGAGGGAGAAGTTGCACGGTCCAGACAGCGAGCGAGTGCGGACCTG GCAGCGAGGGGCTTTCCACCGGCCAGTGCTGGGGGGCTTTCGGGAAAGCCGGGCTGCAGAGGATGGTTTC CGTAAAGCTCAGAAGCCCTGGCTAAAGAGGCTGAAGGAGGTTGAGGCTTCTAAGAAGAGCTACCACACAG CCCGCAAGGATGAGAAGACAGCCCAGACCCGGGAGAGCCACGCGAAGGCAGACAGCTCCATGTCCCAGGA GCAGCTTCGAAAATTACAGGAGCGGGTGGGCCGCTGCACCAAGGAGGCAGAGAAGATGAAAACCCAGTAT GAGCAGACCCTGGCCGAGCTAAATCGCTACACCCCACGCTACATGGAGGACATGGAGCAGGCTTTTGAGA GCTGCCAGGCTGCTGAGCGCCAGCGGCTTCTCTTCTTCAAGGATGTCTTGCTCACCCTGCATCAACACCT CGACCTCTCCAGTAGCGACAAGTTCCATGAACTCCATCGAGACCTGCAGCAGAGCATTGAGGCTGCCAGT GATGAAGAGGATCTGCGCTGGTGGCGGAGCACACACGGGCCTGGCATGGCCATGAACTGGCCACAGTTTG AGGAATGGTCGTTAGACACACAGAGAGCCATCAGCCGCAAGGAGAAGGGTGGCCGGAGCCCAGATGAGGT TACTCTGACCAGCATTGTACCGACAAGAGATGGCACCGCACCCCCACCCCAGTCCCCATCGTCCCCGGGT AGCGGGCAGGACGAGGACTGGTCAGATGAGGAGAGTCCCCGGAAAGTTGCCACTGGGGTGAGGGTACGGG CGCTTTATGACTATGCTGGCCAGGAGGCTGATGAGCTGAGCTTCCGAGCAGGGGAAGAGTTGCTGAAGAT GAGCGAGGAGGATGAGCAGGGCTGGTGCCAGGGCCAGCTGCAGAGTGGCCGCATTGGTCTGTACCCTGCC AATTACGTAGAATGTGTGGGCGCCTGAGTGCCCTGACAGCCCTACCAAGGCGTCTCTCTGACCTGGGCCA GAGCTTAACCCTTCCTGGACTGCTGGACCTGAAGGCCCTGAACCATGTGCTGCTGCTGTCTCTCTTTTCC CACAGGAGGAAGTCTCAGGGCCCATGAAGGGCAGGGGTCTGTGTCTAGAGTTGGAGGTTGGAATGATGGA GGGTCGAGAGGTGCCCGAGCCTCCTCGGGAACATTTGGGTCATCCCAGAGTGGGAGGATCCAGCTCCTAA ACTGTTTAGGAGTTCCTGGGGTGAGGTTGGGGAGAATGTAGTTTGAGGCTGGCAGCACCTTCTTTTGTGA CTTGTAGTGTGCATTAAATTTGATTAAGAATAAAAACCCCATCAAGTGTAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	NM_030880
Insert Size	1275 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC003884, AAH03884
RefSeq Size	1814 bp
RefSeq ORF	1275 bp
Locus ID	80708
UniProt ID	Q99JB8
Gene Summary	Plays a role in endocytosis and regulates internalization of plasma membrane proteins. Overexpression impairs internalization of SLC2A1/GLUT1 and TRPV4 and increases the levels of SLC2A1/GLUT1 and TRPV4 at the cell membrane. Inhibits the TRPV4 calcium channel activity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1. Variants 1 through 4 encode the same protein.

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