LIM kinase 2 (LIMK2) (NM_016733) Human 3' UTR Clone

CAT#: SC215766

3' UTR clone of LIM domain kinase 2 (LIMK2) transcript variant 2b for miRNA target validation



Need custom modification / cloning service?
Get a free quote

CNY 5,795.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (2)
Firefly luciferase assay kit, 150 assays
    • 1 kit

CNY 1,520.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

Specifications

Product Data
Product Name LIM kinase 2 (LIMK2) (NM_016733) Human 3' UTR Clone
Vector pMirTarget
ACCN NM_016733
Insert Size 1669 bp
Sequence Data
>SC215766 3’UTR clone of NM_016733
The sequence shown below is from the reference sequence of NM_016733. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
TACGGCCTGACCCGGGACTCACCTCCCTAGCCCTGGCCCAGCCCCCTGCAGGGGGGTGTTCTACAGCCA
GCATTGCCCCTCTGTGCCCCATTCCTGCTGTGAGCAGGGCCGTCCGGGCTTCCTGTGGATTGGCGGAAT
GTTTAGAAGCAGAACAAGCCATTCCTATTACCTCCCCAGGAGGCAAGTGGGCGCAGCACCAGGGAAATG
TATCTCCACAGGTTCTGGGGCCTAGTTACTGTCTGTAAATCCAATACTTGCCTGAAAGCTGTGAAGAAG
AAAAAAACCCCTGGCCTTTGGGCCAGGAGGAATCTGTTACTCGAATCCACCCAGGAACTCCCTGGCAGT
GGATTGTGGGAGGCTCTTGCTTACACTAATCAGCGTGACCTGGACCTGCTGGGCAGGATCCCAGGGTGA
ACCTGCCTGTGAACTCTGAAGTCACTAGTCCAGCTGGGTGCAGGAGGACTTCAAGTGTGTGGACGAAAG
AAAGACTGATGGCTCAAAGGGTGTGAAAAAGTCAGTGATGCTCCCCCTTTCTACTCCAGATCCTGTCCT
TCCTGGAGCAAGGTTGAGGGAGTAGGTTTTGAAGAGTCCCTTAATATGTGGTGGAACAGGCCAGGAGTT
AGAGAAAGGGCTGGCTTCTGTTTACCTGCTCACTGGCTCTAGCCAGCCCAGGGACCACATCAATGTGAG
AGGAAGCCTCCACCTCATGTTTTCAAACTTAATACTGGAGACTGGCTGAGAACTTACGGACAACATCCT
TTCTGTCTGAAACAAACAGTCACAAGCAAAGGAAGAGGCTGGGGGACTAGAAAGAGGCCCTGCCCTCTA
GAAAGCTCAGATCTTGGCTTCTGTTACTCATACTCGGGTGGGCTCCTTAGTCAGATGCCTAAAACATTT
TGCCTAAAGCTCGATGGGTTCTGGAGGACAGTGTGGCTTGTCACAGGCCTAGAGTCTGAGGGAGGGGAG
TGGGAGTCTCAGCAATCTCTTGGTCTTGGCTTCATGGCAACCACTGCTCACCCTTCAACATGCCTGGTT
TAGGCAGCAGCTTGGGCTGGGAAGAGGTGGTGGCAGAGTCTCAAAGCTGAGATGCTGAGAGAGATAGCT
CCCTGAGCTGGGCCATCTGACTTCTACCTCCCATGTTTGCTCTCCCAACTCATTAGCTCCTGGGCAGCA
TCCTCCTGAGCCACATGTGCAGGTACTGGAAAACCTCCATCTTGGCTCCCAGAGCTCTAGGAACTCTTC
ATCACAACTAGATTTGCCTCTTCTAAGTGTCTATGAGCTTGCACCATATTTAATAAATTGGGAATGGGT
TTGGGGTATTAATGCAATGTGTGGTGGTTGTATTGGAGCAGGGGGAATTGATAAAGGAGAGTGGTTGCT
GTTAATATTATCTTATCTATTGGGTGGTATGTGAAATATTGTACATAGACCTGATGAGTTGTGGGACCA
GATGTCATCTCTGGTCAGAGTTTACTTGCTATATAGACTGTACTTATGTGTGAAGTTTGCAAGCTTGCT
TTAGGGCTGAGCCCTGGACTCCCAGCAGCAGCACAGTTCAGCATTGTGTGGCTGGTTGTTTCCTGGCTG
TCCCCAGCAAGTGTAGGAGTGGTGGGCCTGAACTGGGCCATTGATCAGACTAAATAAATTAAGCAGTTA
ACATAACTGGCAA
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Reference Data
RefSeq NM_016733.3
Summary There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Locus ID 3985
MW 61.5
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Customer Reviews 
Loading...