E2 230K (UBE2O) (NM_022066) Human 3' UTR Clone

Specifications

Product Data
Product Name	E2 230K (UBE2O) (NM_022066) Human 3' UTR Clone
Vector	pMirTarget
Synonyms	E2-230K
ACCN	NM_022066
Insert Size	1517 bp
Sequence Data	Insert Sequence (showhide) >SC214657 3’UTR clone of NM_022066 The sequence shown below is from the reference sequence of NM_022066. The complete sequence of this clone may contain minor differences, such as SNPs. Blue=Stop Codon Red=Cloning site GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC GGCATGCCGGAGTGCACAGAGGACAAGTAGCTGCCAGGCACAGAGGAAAGAGCATCACCGTGGGAGAGG CCAGCCGCCGCCTGCTCACTCCCCCCCGGAATCACCCCTCTTCCCATGCCCCTCTGTCCCCACTGCAAA CCCACTGCCCTCTTCTCCCCAAGGTGAGTTTGATGCTGAAGTGCAAGAAGTGTGTTGAGATGCTGCCGT TTCTATTTTGAAGCGAGCTTTCAACAGGCGGGTCCCCTGTGGCAAAGAAAATCGGAACCCTGTTGCCGA TTTTCCATTTGTCACCCCAGCAGAATGTCCGGCACTTGCTCCCTTGCTGCCCCTTCTCAGGTCAGAGGC GGGTGTTCCAGGGCCTGCCGCGGGGCTCTCTGGGCCGGTTCCCTGCAGACCCGCAGGAGAGCACATGTG CCTTGCATGAAGTGTGGGTTGCGCCAACAATTCCCCTGGTCCCTTTCAACCTGTTTAGTTCAACTCAAG CCTCCCTGTGTCCCAGACCCTCCTGCTGCCACCACCACCCAGGTCCTCCCTAGTCCTCCAGCGTCAACA CTATCCCTTGGGAGTTGTAGCTGCTGTCACTGACTCCCGGCTATACATGGCCTGTCGACCACGTTATAG CCCTCAGGCCTGTTGAACTTGCTCTCTAAGAGAGGTTGGGACCAGGCTAGGTTCCGGGTGACGCCCAGG AGAGGTGGTGGCCTTCACACATGCACATGGAGTTGAGGACCAGGGAGCTGCAGGGAAAGCAACAGCTAT AGGTGCCTTGCTCTTCTGTCGGAGGCTGCTGGGGGCAAGAGCAGCTGCACAAGGCCAGGGCAAGTGCTA GGGCCCCTCCCCCATCACATGGTCACACTGGGACAGGCGTGCAGCTCACTGAACTCCAAGCGAGCCAGC CCTCTCTTGGACTAGAAGGCCTACTGTCAGCCCTTCGCTTACAAACTGCAGGCTCAATCCGAAGGGGAC GGCCGGCGGGGGCTCTCCTAGTGCCCAGAGACAGGCCCAGAGGTTTACAAGTTTTCTAAGCTTTTGATA ATGTGAAGCTCCAGGCCGAGAGGATGCTGTTGAGCACATTGCAGCTATGTAATTTTTGGTGTATGTATG TAATATTTAAGGTTGGAAAAAAAACTCAAAAGCAAAGATATTAACTCTTATTAGAAAAAAAGACAAAAA AAAAGCCAAAGCATGATGCGTCTTGTCAGCCTTAAGTGGGCTCCACACCTGTGCTGTGCTGTGACCGCC CAGCCAGCAGAGCTGCGGGAGGATGGAGCCGGACCACACACCGTGGCATTTGGAACCGAGTCGGTATCT TGTTTGAGAAACACCCGGAGTGACTGGTGGGGCTGTGCTTCCCAGTGCATTGTACATGTGGAGATGTGA ATGCCTACTGCTTACGATATCTGTATAAAGTGCTGTGTGATTAAACTTTTTTTTACTTGCATTTTTTTT TTTTTTTTTTTTTGTGTTGGCTCATTAGCAAGACAAGGTGACACCATTAAACCTCCCTCTGCCATTCA ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites	SgfI-MluI
OTI Disclaimer	Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Reference Data
RefSeq	NM_022066.4
Synonyms	E2-230K
Summary	E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853).[UniProtKB/Swiss-Prot Function]
Locus ID	63893
MW	55.3

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