PUS7 (NM_019042) Human 3' UTR Clone

CAT#: SC213654

3' UTR clone of pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7) for miRNA target validation



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CNY 4,845.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (2)
Firefly luciferase assay kit, 150 assays
    • 1 kit

CNY 1,520.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

Specifications

Product Data
Product Name PUS7 (NM_019042) Human 3' UTR Clone
Vector pMirTarget
Synonyms IDDABS
ACCN NM_019042
Insert Size 1317 bp
Sequence Data
>SC213654 3’UTR clone of NM_019042
The sequence shown below is from the reference sequence of NM_019042. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
ACGCAGCTGAATACAACCTGGCTTCGCTGAGCAGTACCTTGTCCACAGATTAGAAAACGTACACAAGTG
TTTGCTTCCTGGCTCCCTGTGCATTTTTGTCTTAGTTCAGACTCATATATGGATTTCAAATCTTTGTAA
TAAAAATTATTTGTATTTTTAAGTTTTTATTAGCTTAAAGAAATAATTTGCAATATTTGTACATGTACA
CAAATCCTGAGGTTCTTAATTTTAGCTCAGAATATAAATTAGTCAAAATACACTTCAGGTGCTTAAATC
AGAGTAAAATGTCAGCTTTACAATAATAAAAAAAGGACTTTGGTTTAAAGTAGCAGGTTTAGGTTTTGC
TACATTCTCAAAAGACAGCAGGAGTATTTGACACATCTGTGATGGAGTATACAACAATGCATTTTAAGA
GCAAATGCAACAAAACAAATCTGGACTATGGATAAATAATTTGAGAGCTGCCACCCACAAATATAAATA
CAGTACTCATGCTGACTGAAATAATAAGACATCTACAAATTTATAAACAAAAAGTGATTGTCATTATCC
TGCTTATGTACTAGATTCAGGCAAGCATTATAGACTTTTTGGTTGCGGTGGCTTTTGCATTTATATTAT
CAATGCCTTGCAGGAACGTTGCATTGATAGGCCCATTTTATTTTTTTATTTTTTTTTTCGAGACAGGAT
CTCACTCTGTAGCACAGGCTGGATTGCAGTGCAATCCTGCAATTCTCAATCTTGCACTGCAGCCTCGAC
CTCCCAGGCTCCAGTGACTCTCCCACCTCAGCCTCCTAAGTAGCTGGGAGTACAGGCGCGCACCACCAC
GCCTAGCTGATTTTTGTATTTTTTTGTAGAGACGGGGGTTTGGCCATGTTGCCGAGGCTAACTCCTGGG
ATTACAGGCATGAGCTGTGCTGGCCGGGTTTTTTTTTCTTGATGTAAACGTGTACAGCTGTTTTATTAG
TTAAGGTCTAATTTTTACTCTAGGTGCCTTTTATGTTCAGAACTCTTTCCACTGGACTGGTATTTGCTC
AAAAATAAATAATGGTAGAGAAGAAAACTATAAAAATGGACAAGGCTTTCTTCTATCAGTAGCGTTTAC
CCTTTGTCACCAGTGGCTTTGGTATTTCCATGTCTGGCATTGCATAAACTTCTCTGGTGTGAAAGGATA
AATATGCCTTTCTAAAGTTGTATATCAAAATTGTATCAATTTTTATTTTCTATGATTTCTAGAAACAAA
TGTAATAAATATTTTTAAAATCTCCTTTCTACTGGTTATGTAAATAAATCAAATAAATATATCAAAATG
AGTGCA
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Reference Data
RefSeq NM_019042.5
Synonyms IDDABS
Summary Pseudouridylate synthase that catalyzes pseudouridylation of RNAs (PubMed:28073919, PubMed:29628141). Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs): pseudouridylated tRFs inhibit translation by targeting the translation initiation complex (PubMed:29628141). Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3' (PubMed:28073919). In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types (PubMed:29628141).[UniProtKB/Swiss-Prot Function]
Locus ID 54517
MW 51.5
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