ASAH2 (NM_001143974) Human 3' UTR Clone

CAT#: SC200461

3' UTR clone of N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2) transcript variant 2 for miRNA target validation



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CNY 4,845.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (2)
Firefly luciferase assay kit, 150 assays
    • 1 kit

CNY 1,520.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

Specifications

Product Data
Product Name ASAH2 (NM_001143974) Human 3' UTR Clone
Vector pMirTarget
Synonyms BCDase; HNAC1; LCDase; N-CDase; NCDase
ACCN NM_001143974
Insert Size 2000 bp
Sequence Data
>SC200461 3’UTR clone of NM_001143974
The sequence shown below is from the reference sequence of NM_001143974. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
TCCCCGGCTTTTGAAGTTGTAACTATTTAGTGAAAAGTTGATAGATCATTTAAAGAACAGCTTTACTCT
CTACACATTATATAAGTGATTTCAAATGAATGTGAACTAGTGAACTACCATGTTGACTTCTATAATCGT
CCCTGTTTGGGGACAGATAGTTTACTGCTAATGGGGTGGAGGGGTGTGTGTGTGTGTGTGTGTGTGTGT
GTGTGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGTTTGTCCCATATATC
TTGCTCCAGCAGCCATATATCTTGTGGTCTACAGCCTAAAGCATGATTTCCCTTGAAGTCTTGGGGTTG
TTTAAAGGAGAGTCCCTTCAATATAAAACCTCTGAAATATTAGTGAGAATGGCTCACTAATGTGAACAA
TGTTTAAATTATTTATTTATATATAGAATTACTGAATATTAGTACTGGGAAAATTTATAGAAATCATCT
AGTCTTACCCTTCATCTTACATATAAGAAAAATGGTCTTTTCTTCTAATCACATTTACAAAATATGATA
TAAACCTTGACCATGAATGTATGAGCCTAATTAGAGAAACAGAAAATCAGCATGTCAGTTTTCCTTCAT
TCAAAATAACATAGTCTTTCTAAGCAGTCATTCTGGAGTTAACATGCCTAGTTCAGAGCCGCTGTGGAT
GCTCAAATCATTTCTGGAATTGCCTTCAGGACACAATTATGAGAAAGTCAGACTTCCAATATTTTGATC
ATGCCTTGTGTTTTCCTAAGTGTCCTTATCCCACTGGATTGTGTCTCTTACTCCCAAGACTTATTCCAA
ATGATTTTTTGACTCTTTTCATTAATCAAATTCACCCCAAACCAGAAAGTTTTGCCATTAGTGTAGATA
TTAAAAATATTACTGGCTGGGAAAGCACTCCTCAAAGCAGAGGTTCTAAATTAATTTATCTGTTCTTTT
ACCATTTGCTCACTCACTCACTCATGGACCCATTCAATCATAGAATATTTAAGATTTATATGCCCAGAA
TTATAATAGTGCCTAGGCCTTGATAATTAGTGAATTTGTACTTGGGGTGAATGTGTAAGTGTCAAAAGG
TAACTATTATGAAGGAGAAAATAATCGATTTAATATGTAAATTCTGGGGAAGCATTTAAAAAATCAGTA
ATCTTCAAGTTATATCTTGTGTATCATCTGTGTTGGATACTCTAATTTGGGGGGAAAGAGGCTCCAGAC
AGTTTGCTAAATACTATTTTGAAAGTTATTTTTGGGCATTGTAAAATACCTTGCTTCATACCCCAAAGA
ATAAGATATGACAGTCTGACATCGATTGTCTGAAAGTACTAATTGCATTTAAAATTCTTTAAGTTAACT
ACAACCTCCCAGACTGCAAAGCAAATTTCCAAACCCCAGGTCATACTTTTGTATGTATGTAAAAGCTTT
AGCATTTTAGGGAAAAAAATCAATCTGTATTTTAAAAATAAACTTTGAGGTTTGAAAATAATAACAATA
TTCATAATGGTAGTATTAACATCTATTATGTATAACTTAATCTCAACATAATTTATTGAATTAGAATGA
ACCTGGAACACTTTTTGATGAATTGGTATCCTAGAGGCTGTTTCCTTGGGCATTAACTGCTGGAGGATT
AGGTAATCAGGGATTCCAAGTGTTGTGATCTTTTTTCTGATGATGCCACAGTCTTCAGTGTAAAAGTTC
AGAAGCCTAAAAGAACAAGTGATCCTGAAAGTAGCTGATTCCAGACAAGAAAATTATATTAGAACTTAT
GATATGGTTTTTTAAAGTATTTGTTATTACTCAGAAGGGTTCATGAATCAGTTTCCACTACCTCTATTC
CTTCTATTGAAGCTTTCCTCAAATCTACTTGTGATTCCTGATAGCTCACTGGCAACTGTACCAAGGTGG
CCACTGTATCATCTACTGATCCTACTGCACCTAGCACTCTTGGCTCCAGATAACGCACAGAAACCTAT
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Reference Data
RefSeq NM_001143974.3
Synonyms BCDase; HNAC1; LCDase; N-CDase; NCDase
Summary Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008]
Locus ID 56624
MW 77
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