Sheep IL6 ELISA KIT (96 T)

Specifications

Product Data
Description	Sheep IL-6 Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	78.1-5000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant Sheep IL-6.
Sensitivity	15 pg/mL
Reactivity	Sheep
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,5000pg/ml upon reconstitution,2 vials lyophilized, Biotin-Conjugated antibody,2 vials Concentrated Streptavidin-HRP,1 vial Standard /sample Diluent,1 bottle Biotin-Conjugate antibody Diluent,1 bottle Streptavidin-HRP Diluent,1 bottle 20 x Wash Buffer Concentrate,1 bottle Substrate Solution,1 bottle Stop Solution,1 bottle Plate Cover Seals,4 pieces
Background	IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine and is produced by T cells, macrophages, fibroblasts, osteoblasts, endothelial and other cells. IL-6 induces proliferation and differentiation and acts on B cells, T cells, thymocytes, and others. IL-6 is one of the most important mediators of fever and of the acute phase response. In the muscle and fatty tissue, IL-6 stimulates energy mobilization that leads to increased body temperature. IL-6 can be secreted by macrophages in response to specific microbial molecules, referred to as pathogen associated molecular patterns (PAMPS). IL-6 in concert with TGFβ is important for developing Th17 responses. IL-6 binds to IL-6Rα that through association induces gp130 homodimerization. gp130 homodimerization triggers the Jak/STAT cascade and the SHP2/Erk Map kinase cascade. IL-6 also forms a complex with an IL-6Rα splice variant that is non-membrane associated. The IL-6/soluble IL-6Rα complex can then activate the gp130 signaling pathway on cells that express gp130 but not IL6Rα. IL-6 is relevant to many disease processes such as diabetes, atherosclerosis, depression, Alzheimer's Disease, systemic lupus erythematosus, prostate cancer, breast cancer, and rheumatoid arthritis.

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