Mouse MOK protein kinase (MOK) ELISA KIT (96 T)

Specifications

Product Data
Description	Mouse RAGE Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	62.5-4000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant mouse RAGE.
Sensitivity	30pg/mL
Reactivity	Mouse
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,4000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Receptor for Advanced Glycation End product (RAGE) is an approximately 50 kDa type I transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily. RAGE plays important roles in several pathological processes including inflammation, diabetes, cancer, and Alzheimer’s disease (AD). RAGE consists of a 319 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 41 aa cytoplasmic domain. Within the ECD, mouse RAGE shares 79% and 91% aa sequence identity with human and rat RAGE, respectively. Alternative splicing of mouse RAGE generates multiple additional isoforms that are truncated at various points within the ECD or carry internal deletions. A soluble form of RAGE (sRAGE) can also be generated by metalloproteinase-mediated cleavage of the ECD. The membrane-bound fragment remaining after ECD shedding can be cleaved by gamma-secretase to release the intracellular domain. RAGE is expressed in the central nervous system (CNS) during development as well as in adult endothelial cells, smooth muscle cells, pericytes, monocytes, and neurons. RAGE binding to S100A1, EN-RAGE/S100A12, or S100B induces inflammatory immune cell adhesion and infiltration as well as vascular smooth muscle proliferation, neointimal expansion, and atherosclerotic plaque development. RAGE also cooperates with TLR9 in the B cell and dendritic cell inflammatory response to complexes of HMGB1 and CpG DNA. In cancer, RAGE binding to HMGB1, S100A8, or S100A9 promotes tumor growth and metastasis in addition to inflammatory cell infiltration. sRAGE functions as a sink for RAGE ligands and lessens the severity of these processes
Gene Symbol	MOK

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