Mouse Lep ELISA KIT (96 T)

Specifications

Product Data
Description	Mouse Leptin Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	125-8000 pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant mouse Leptin.
Sensitivity	60pg/mL
Reactivity	Mouse
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,8000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Mouse Leptin is a protein product of the mouse obese gene. Mutant mice that lack functional Leptin have been found to be obese, diabetic, infertile and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse and rat cells. Mature mouse Leptin shares approximately 96% and 84% amino acid (aa) sequence identity with the rat and human proteins, respectively. Mouse Leptin cDNA encodes a 167 aa residue protein with a 21 aa residue signal sequence that is cleaved to yield the 146 aa residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissues and placenta. A high-affinity receptor for Leptin (OB-R) with homology to gp130, G-CSF receptor, and LIF receptor has been cloned. Multiple isoforms of OB-R, including a long form (OB-RL) with a large cytoplasmic domain capable of signal transduction, and several receptor isoforms with short cytoplasmic domains (OB-RS) lacking signal transducing capabilities, have been identified. An OB-R transcript lacking a transmembrane domain and potentially encoding a soluble form of the receptor has also been described. OB-RL transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-RS transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney and primitive hematopoietic cell populations. OB-R has been shown to be encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have long been known to exhibit an obesity phenotype almost identical to the phenotype of ob/ob mice.
Gene Symbol	Lep

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.