Mouse Tnfsf13b ELISA KIT (96 T)

Specifications

Product Data
Description	Mouse BAFF Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	62.5-4000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant mouse BAFF.
Sensitivity	30pg/mL
Reactivity	Mouse
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,4000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	B-cell activating factor (BAFF), also known as BLyS, TALL-1, and THANK, is a TNF superfamily member (TNFSF13B) best known for its role in the survival and maturation of B cells. Mouse BAFF is 32 kDa type II transmembrane protein, and an 18 kDa form can also be shed into the serum. The N-terminal side of the mouse BAFF TNF homology domain contains a furin cleavage site (RNRR) responsible for the release of soluble BAFF. A conserved alternatively spliced isoform termed BAFF has also been described. It can form heteromultimers with BAFF and may act to negatively regulate BAFF secretion. BAFF is produced by several cell types and tissues including monocytes, macrophages, neutrophils, dendritic cells, T lymphocytes, spleen, lymph node, and bone marrow. It is thought to exist as a homotrimer, but it may also exist as a heteromer in association with related TNFSF member APRIL.
Gene Symbol	Tnfsf13b

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