Human Eotaxin 2 (CCL24) ELISA KIT (96 T)

Specifications

Product Data
Description	Human CCL24 Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	15.62-1000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant human CCL24.
Sensitivity	8pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,2000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Eotaxin-2, a CC chemokine also known as CCL24, MPIF-2, or Ckβ-6, was initially discovered through a large-scale cDNA sequencing effort of an activated monocyte library, and was independently characterized by three separate groups reporting in 1997. Mature Eotaxin-2 is a glycosylated 93 amino acid (aa) residue protein generated following removal of a 26 aa signal peptide. It has a predicted molecular weight of 10.6 kDa. Eotaxin-2 exhibits relatively low aa identity (32%-40%) with other known CC chemokines having overlapping functional profiles (i.e. Eotaxin, MCP-3, MCP-4, and RANTES). The 3-dimensional structure of Eotaxin-2 confirms the existence of an N-loop structure important for receptor binding and an N-terminal α-helix implicated in receptor signaling. Eotaxin-2 and Eotaxin-3 are the only known chemokines to map to chromosome 7.
Gene Symbol	CCL24

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