Human IL1 alpha (IL1A) ELISA KIT (96 T)
CNY 3,200.00
货期*
3周
规格
Specifications
Product Data | |
Description | Human IL-1sRII ELISA KIT |
Size | 96 T |
Format | 96-well strip plate |
Assay Type | Solid Phase Sandwich ELISA |
Assay Length | 4 hours |
Signal | Colorimetric |
Curve Range | 31.25-2000pg/ml |
Sample Type | Serum, plasma, Cell culture supernatant |
Sample Volume | 100 uL |
Specificity | This assay recognizes both natural and recombinant human IL-1sRII. |
Sensitivity | 15 pg/ml |
Reactivity | Human |
Interference | No significant interference observed with available related molecules. |
Components |
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Background | Both IL-1α and IL-1sRII are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17 kDa. IL-1α and IL-1sRII exert their effects by binding to specific receptors. Two distinct receptors have been identified that bind both forms of IL-1. Common to each is an extracellular immunoglobulin-like (Ig-like) domain that places both IL-1 receptors (IL-1 R) in the type IV cytokine receptor family. The first IL-1 receptor (IL-1 RI) is an 80 kDa transmembrane glycoprotein that has been isolated from astrocytes, chondrocytes, endothelium, fibroblasts, keratinocytes, neurons, oocytes, pancreatic β-cells, smooth muscle cells, and T cells. The second type of IL-1 R (IL-1 RII) is a 60-68 kDa transmembrane glycoprotein which has been found on B cells, keratinocytes, monocytes, neutrophils, and T cells. The two receptors show approximately 28% homology in their extracellular domains but differ significantly in their cytoplasmic regions where IL-1 RI contains 215 amino acid (aa) residues compared to only 29 for IL-1 RII. Depending on species and cell type, the two receptors show differential binding characteristics for IL-1α and IL-1sRII. In general, IL-1α binds better to the type I receptor, and IL-1sRII binds better to the type II receptor. |
Gene Symbol | IL1A |
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