One-step HIV-1 p24 Protein ELISA Kit 1 x 96

Specifications

Product Data
Description	One-step HIV-1 p24 Protein ELISA Kit 1 x 96
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich
Assay Length	1.5 hours incubations; 10 minutes washing and analyzing samples
Signal	Colorimetric
Curve Range	125-2000 pg/mL
Sample Type	Cell cuture supernatant, lentiviral sample
Sample Volume	1µl of lentiviral sample or 90µl diluted sample
Specificity	This kit is used for the detection of HIV-1 p24 protein
Sensitivity	4.18pg/mL
Reactivity	Virus
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	HIV-1 p24 Antibody Coated 96-well Plate in foil pouch with desiccant|1 plate Recombinant P24 Standard (100ng/ml)|0.05 mL 1x P24 Detection Antibody-HRP Conjugate|2 mL Assay Buffer|60 mL Lysis Buffer2 mL Wash Buffer Concentrate 20X|30 mL TMB Substrate|12 mL Stop Solution|12 mL
Background	The gag protein p24 (MW 24kD) is a major structural protein of the HIV capsid, which is required for virus particle assembly . There are approximately 2000 p24 molecules per virus particle, or at a molecule weight of 24 kDa, about 104 virus particles per picogram of p24. The onset of symptoms of AIDS correlates with an increased level of virus and p24 in the blood. HIV-1 p24 measurement is commonly used as an indicator of HIV-1 infection and viral load. In gene therapy, HIV-derived lentiviral vectors are commonly used to deliver transgenes to mammalian cells. Quantitative detection of the HIV-1 p24 has become a standard method for measuring lentiviral transduction efficiency.

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