Human Von Willebrand Factor (VWF) ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	For quantitative detection of human VWF-A2 in cell culture supernates, serum and plasma (heparin, EDTA).
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human VWF-A2
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	312pg/ml-20.000pg/ml
Specificity	This kit is used for quantitative detection of Human VWF-A2
Sensitivity	<50pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti- human VWF-A2 antibody | 1 Lyophilized recombinant human VWF-A2 standard | 20 ng/tube×2 Biotinylated anti- human VWF-A2 antibody | 100 μl(dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC) | 100 μl(dilution 1:100) Sample diluent buffer | 30 ml Antibody diluent buffer | 12 ml ABC diluent buffer | 12 ml TMB color developing agent | 10 ml TMB stop solution | 10 ml Wash Buffer (25x) | 20 ml Adhesive cover | 4
Background	Von Willebrand factor (VWF) is a blood glycoprotein involved in hemostasis. It is mapped to 12p13.31. The VWF gene encodes von Willebrand factor (VWF), a large multimeric glycoprotein that plays a central role in the blood coagulation system, serving both as a major mediator of plateletvessel wall interaction and platelet adhesion, and as a carrier for coagulation factor VIII. VWF released from endothelial cell Weibel-Palade bodies bound particularly avidly to the extracellular matrix. VWF deficiency or dysfunction (von Willebrand disease) leads to a bleeding tendency, which is most apparent in tissues having high blood flow shear in narrow vessels.
Gene Symbol	VWF

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