Human CD28 ELISA KIT (1 x 96 wells)

CAT#: EA102307

For quantitative detection of human CD28 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).



CNY 5,000.00


货期*
3周

规格
    • 1 x 96 wells

Product images

Specifications

Product Data
Description For quantitative detection of human CD28 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Size 1 x 96 wells
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for human CD28
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 62.5pg/ml-4000pg/ml
Specificity This kit is used for quantitative detection of human CD28
Sensitivity <12pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Components
  • 96-well plate precoated with anti- human CD28 antibody | 1
  • Lyophilized recombinant human CD28 standard | 10ng/tube×2
  • Biotinylated anti- human CD28 antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
Background CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co- stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). The CD28 gene is mapped to 2q33-q34. CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily.
Gene Symbol CD28
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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