Human Carbonic Anhydrase II (CA2) ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	For quantitative detection of human CA2 in cell culture supernates, serum and plasma(heparin, EDTA, citrate) and urine.
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human CA2
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	0.78ng/ml-50ng/ml
Specificity	This kit is used for quantitative detection of Human CA2
Sensitivity	<50pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti- human CA2 antibody | 1 Lyophilized recombinant human CA2 standard | 50ng/tube×2 Biotinylated anti- human CA2 antibody | 130μl(dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) Sample diluent buffer | 30 ml Antibody diluent buffer | 12ml ABC diluent buffer | 12ml TMB color developing agent | 10ml TMB stop solution | 10ml Adhesive cover | 4
Background	CA2 is a cytosolic enzyme with the highest activity among all known CAs. The carbonic anhydrases (ACs) form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs relatively slowly in the absence of a catalyst. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. This gene is mapped to 8q22.
Gene Symbol	CA2

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