PAP ELISA Kit
CNY 17,231.00
货期*
9周
规格
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Specifications
Product Data | |
Description | PAP ELISA Kit |
Size | 1 kit |
Format | 96-well strip plate |
Assay Type | Solid Phase Sandwich ELISA |
Assay Length | 3 hours |
Signal | Colorimetric |
Sample Type | Cell culture supernatant, serum, plasma |
Sample Volume | 50 ul |
Specificity | Natural and recombinant human PAP |
Reactivity | Human |
Interference | No significant interference observed with available related molecules. |
Components | See the product information for more details |
Background | The plasmin-a2-antiplasmin (PAP) micro ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. During the first incubation step the PAP present in the sample binds to the monoclonal antibodies (PAP-6) coated on the surface of the microtitration plate. Unbound fragments are removed by washing and, in a second reaction, peroxidase-conjugated antibodies to plasminogen are bound to the free PAP determinants. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of PAP in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of PAP in the donor sample. |
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