Human Mitochondrial Antibody (MA) ELISA kit

Specifications

Product Data
Description	Human Mitochondrial Antibody (MA) ELISA kit
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Sample Type	Serum
Sample Volume	10 µl/well
Specificity	This kit is used for quantitative detection human Mitochondrial Antibody (MA)
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	1. Microwells coated with Mitochondrial antigen: 12x8x1 2. Sample Diluent: 1 bottle (ready to use): 22 ml 3. Calibrator: 1 Vial (ready to use): 1ml 4. Positive Control: 1 vial (ready to use): 1ml 5. Negative Control: 1 vial (ready to use): 1ml 6. Enzyme conjugate: 1 bottle (ready to use): 12ml 7. TMB Substrate: 1 bottle (ready to use): 12ml 8. Stop Solution: 1 bottle (ready to use): 12ml 9. Wash concentrate 20X: 1 bottle: 25ml
Background	Mitochondrial Antibodies (MA) are directed against the E2 subunit of the pyruvate dehydrogenase enzyme complex located at the inner mitochondrial membrane (PDC-E2), the E2 subunit of the branched chain 2- oxo acid dehydrogenase complex (BCOADC-E2), the E2 subunit of the 2-oxo-glutarate dehydrogenase complex (OGDC-E2), protein X, and PDC-Ela and PDC-E1. MA are found in ~95% of patients with primary biliary cirrhosis (PBC). MA in low titers are common in chronic active hepatitis and their presence does not preclude response to corticosteroids. MA disappear in about one month after orthotopic liver ransplantation (OLT) and decrease with cyclosporine treatment which might be useful in PBC. MA are found in <1% of apparently healthy Caucasoid adults. Approximately 3% of patients with PBC have scleroderma, usually of the CREST syndrome variety. In addition, MA reactive with the PDC-E2 complex are found in some patients with CREST or diffuse scleroderma, sometimes in the absence of overt liver disease. Scleroderma typically precedes PBC in those patients with both diseases.

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