Human Adrenocorticotropic hormone (ACTH) ELISA kit

Specifications

Product Data
Description	Human Adrenocorticotropic hormone (ACTH) ELISA kit
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	6.4-483 pg/ml
Sample Type	Serum
Sample Volume	200 µl/well
Specificity	This kit is used for quantitative detection human Adrenocorticotropic hormone (ACTH)
Sensitivity	6.4 pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	Microwells coated with Streptavidin: 12x8x1 Biotinylated ACTH Antibody (Reagent 1): 2.7 ml Peroxidase (Enzyme) labeled ACTH Antibody (1 Vial): 2.7 ml Wash Concentrate (1 Vial): 30 ml TMB Substrate (1 Vial): 15 ml Stop Solution (1 Vial): 20 ml Calibrators (5 Vials): 2 ml Zero Calibrator (1 Vial): 4 ml Controls 1 & 2 (CTRL) (2 Vials): 2 ml
Background	Adrenocorticotropic Hormone (ACTH) is a 39-amino acid peptide hormone (MW = 4500) secreted by the pituitary to regulate the production of steroid hormones by the adrenal cortex. ACTH increases the synthesis and release of all adrenal sterioids, aldosterone, cortisol and adrenal androgens. It is the principal modulator of cortisol, the most important glucocorticoid in man. As the cortisol level in blood increases, release of ACTH is inhibited directly at the pituitary level. Through this same mechanism, decreasing cortisol levels lead to elevated ACTH levels. In healthy individuals, ACTH reaches a peak in the early morning (6:00 - 8:00 hour) and levels become lowest late in the day and near the beginning of the sleep period. Stress may also override the diurnal variation. Plasma ACTH assays are useful in the differential diagnosis of pituitary Cushing’s disease, Addison’s disease, autonomous ACTH producing pituitary tumors (e.g. Nelson’s syndrome), hypopituitarism with ACTH deficiency and ectopic ACTH syndrome. Primary adrenocortical insufficiencies, Addison’s disease. Hypopituitarism with ACTH deficiency, which is secondary adrenocortical insufficiency, is characterized by low plasma ACTH and cortisol concentrations, and a subnormal, but usually distinct adrenal response to stimulation with synthetic ACTH (Cortrosyn)

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