Human JAM-A ELISA Kit

Specifications

Product Data
Description	Human JAM-A ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human JAM-A
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	46.9pg/ml-3000pg/ml
Sample Type	Cell culture supernates and serum.
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human JAM-A
Sensitivity	<10pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- human JAM-A antibody 1 Lyophilized recombinant human JAM-A standard 10ng/tubex2 Biotinylated anti- human JAM-A antibody 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC) 130ul(dilution 1:100) Sample diluent buffer 30 ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml
Background	Junctional adhesion molecule A(JAM-A) is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that colocalizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor. JAM-A has a nonredundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.
Gene Symbol	F11R

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