Human PCSK9 ELISA Kit

Specifications

Product Data
Description	Human PCSK9 ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human PCSK9
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	156pg/ml-10,000pg/ml
Sample Type	Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human PCSK9
Sensitivity	<10pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- human PCSK9 antibody | 1 Lyophilized recombinant human PCSK9 standard 10ng/tube | 2 Biotinylated anti- human PCSK9 antibody 130ul (dilution 1:100) | 1 Avidin-Biotin-Peroxidase Complex(ABC) 130ul (dilution 1:100) | 1 Sample diluent buffer 30ml | 1 Antibody diluent buffer 12ml | 1 ABC diluent buffer 12ml | 1 TMB color developing agent 10ml | 1 TMB stop solution 10ml | 1 Wash Buffer (25 x) 20ml | 1 Plate Sealers | 4
Background	Proprotein convertase subtilisin/kexin type 9, also known as PCSK9, is an enzyme that in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. By genomic sequence analysis, the PCSK9 gene was mapped to chromosome 1p32. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A(EGF-A) domain of the low-density lipoprotein receptor(LDLR), inducing LDLR degradation. Reduced LDLR levels result in decreased metabolism of low-density lipoproteins(LDL), which could lead to hypercholesterolemia. PCSK9 may also have a role in the differentiation of cortical neurons.
Gene Symbol	PCSK9

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