Human ADAM12 ELISA Kit

Specifications

Product Data
Description	Human ADAM12 ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human ADAM12
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	156 pg/ml-10,000 pg/ml
Sample Type	Cell culture supernates, serum, plasma(heparin) and urine.
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human ADAM12
Sensitivity	<10 pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti-human ADAM12 antibody | 1 Lyophilized recombinant human ADAM12 standard | 10ng/tube x 2 Biotinylated anti-human CCL21 antibody | 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) | 130ul (dilution 1:100) Sample diluent buffer | 30ml Antibody diluent buffer | 12ml ABC diluent buffer | 12ml TMB color developing agent | 10ml TMB stop solution | 10ml Plate Sealers | 4 Wash Buffer (25x) | 20 ml
Background	ADAM12(Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM(a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.
Gene Symbol	ADAM12

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