Human MMP-8 ELISA Kit

Specifications

Product Data
Description	Human MMP-8 ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human MMP-8
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	156pg/ml-10,000pg/ml
Sample Type	Cell culture supernates, serum, plasma(heparin) and saliva.
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human MMP-8
Sensitivity	<10pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- human MMP-8 antibody | 1 Lyophilized human MMP-8 standard 10ng/tubex2 | 1 Biotinylated anti- human MMP-8 antibody 100 ul(dilution 1:100) | 1 Avidin-Biotin-Peroxidase Complex(ABC) 100 ul(dilution 1:100)| 1 Sample diluent buffer 30ml | 1 Antibody diluent buffer 12m | 1 ABC diluent buffer 12ml | 1 TMB color developing agent 10ml | 1 TMB stop solution 10ml | 1 Wash Buffer (25x) 20ml | 1 Plate Sealers | 4 piece
Background	Matrix metalloproteinase 8 (MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase(HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation. The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.
Gene Symbol	MMP8

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