Human MMP-3 ELISA Kit

Specifications

Product Data
Description	Human MMP-3 ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human MMP-3
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	156pg/ml-10,000pg/ml
Sample Type	Cell culture supernates, serum and plasma(heparin).
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human MMP-3
Sensitivity	<10pg/ml
Reactivity	Human
Cross Reactivity	There is cross-reactivates with MMP-10 approximately 2%, and no detectable cross-reactivity with other MMPs.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- human MMP-3 antibody 1 Lyophilized recombinant human MMP-3 standard 10ng/tubex2 Biotinylated anti- human MMP-3 antibody 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) Sample diluent buffer 30ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml
Background	Matrix metalloproteinase-3(MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase(MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54% identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures. MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition. The standard product used in this kit is recombinant human MMP-3, consisting of 460 amino acids with the molecular mass of 52KDa. The detected MMP-3 includes zymogen and active enzyme.
Gene Symbol	MMP3
Standard Curve	Representative standard curve for Human MMP-3 PicoKine ELISA Kit

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