Human MCP-1 ELISA Kit

Specifications

Product Data
Description	Human MCP-1 ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human MCP-1
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	15.6pg/ml-1000pg/ml
Sample Type	Cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine.
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human MCP-1
Sensitivity	<1pg/ml
Reactivity	Human
Cross Reactivity	There is cross-reactivity with human Eotaxin, MCP-3<1%.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- human MCP-1 antibody 1 Lyophilized recombinant human MCP-1 standard 10ng/tubex2 Biotinylated anti- human MCP-1 antibody 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) Sample diluent buffer 30ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml
Background	Monocyte chemoattractant protein-1(MCP-1), a member of the chemokine(chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.
Gene Symbol	CCL2
Standard Curve	Representative standard curve for Human MCP-1 PicoKine ELISA Kit

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