Human IL-1 beta ELISA Kit

Specifications

Product Data
Description	Human IL-1 beta ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human IL-1 beta
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	3.9pg/ml-250pg/ml
Sample Type	Cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human IL-1 beta
Sensitivity	<0.15pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- human IL-1 beta antibody 1 Lyophilized recombinant human IL-1 beta standard 1ng/tube x 2 Biotinylated anti- human IL-1 beta antibody 100ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 100ul(dilution 1:100) Sample diluent buffer 30ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml Plate Sealers 4 x Wash Buffer (25x) 20ml
Background	Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B(NFKB)-regulated genes. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.
Gene Symbol	IL1B
Standard Curve	Representative standard curve for Human IL-1 beta PicoKine ELISA Kit

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