Human ICAM-1 ELISA Kit

Specifications

Product Data
Description	Human ICAM-1 ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human ICAM-1
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	156pg/ml-10,000pg/ml
Sample Type	Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human ICAM-1
Sensitivity	<10pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- human ICAM-1 antibody 1 Lyophilized recombinant human ICAM-1 standard 10ng/tubex2 Biotinylated anti- human ICAM-1 antibody 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) Sample diluent buffer 30ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml Plate Sealers 4 x Wash Buffer 1 Powder Pack for 1000 ml
Background	Intercellular adhesion molecule-1(ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated(LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1(ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen(LFA-1) or macrophage-1 antigen(Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1
Gene Symbol	ICAM1
Standard Curve	Representative standard curve for Human ICAM-1 PicoKine ELISA Kit

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