Mouse MIP-2 ELISA Kit (96-well)

Specifications

Product Data
Description	Mouse MIP-2 ELISA Kit (96-well)
Size	1 x 96 wells
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hour
Signal	Colorimetric
Curve Range	0.02 - 1.0 ng/mL
Sample Type	Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume	20 uL
Specificity	Natural and recombinant Mouse MIP-2 Ligand
Sensitivity	7pg/mL
Reactivity	Mouse
Interference	No significant interference observed with available related molecules.
Components	Aluminium pouches with a Microwell Plate coated with antibody to mouse MIP-2 (8X12) 2 vials mouse MIP-2 Standard lyophilized, 500 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-mouse MIP-2 antibody 2 vials Streptavidin-HRP solution 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 4 pieces Adhesive Films package insert
Background	Mouse macrophage inflammatory protein-2 (MIP-2), also known as CXCL2, was originally identified as a heparin-binding protein secreted by an LPS-stimulated mouse macrophage cell line . A cDNA clone encoding the protein was isolated from this cell line and characterized . Based on its protein and DNA sequences, mouse MIP-2 was classified as a member of the alpha (CXC) chemokine family of inflammatory and immunoregulatory cytokines . Mouse MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature mouse MIP-2. The protein sequence of mouse MIP-2 shows approximately 63% identity to that of mouse KC, another mouse alpha chemokine. Mouse MIP-2 is also 60% identical to human GRO. and GRO.. Based on these protein sequence similarities, it is likely that mouse KC and MIP-2 are homologs of human GRO., . and . chemokines. Since chemokines with protein sequence homology to human IL-8 have not been identified in mice, it has been suggested that the mouse KC and MIP-2 are functional homologs of human IL-8 in mice . A putative mouse homolog of the human IL-8 receptor beta (IL-8 R.) has also been cloned. This receptor shows 71% identity to human IL-8 R. and 68% identity to human IL-8 R.. Both mouse KC and MIP-2 bind mouse IL-8 R. with high affinity . Like human IL-8, mouse MIP-2 exhibits potent neutrophil chemotactic activity and may be a key mediator of neutrophil recruitment in response to tissue injury and infection . Increased MIP-2 expression has been found to be associated with neutrophil influx in various inflammatory conditions .
Gene Symbol	Cxcl2
Standard Curve	Representative standard curve for MIP-2 ELISA. MIP-2 was diluted in serial two-fold steps in Sample Diluent.

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