Human IFN-a ELISA Kit (96-well)

Specifications

Product Data
Description	Human IFN-a ELISA Kit (96-well)
Size	1 x 96 wells
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	15.625-1000pg/ml
Sample Type	Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume	20 uL
Specificity	Natural and recombinant Human IFN-a Ligand
Sensitivity	7pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Aluminium pouches with a Microwell Plate coated with antibody to human IFN-a(8X12) 2 vials human IFN-a Standard lyophilized, 500pg/ml upon reconstitution 2 vials HRP-Conjugate solution 1 bottle Standard /sample Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 4 pieces Adhesive Films package insert
Background	IFN-./. R2, also known as IFNAR2, is a 100 kDa glycoprotein in the class II cytokine receptor family. These proteins form heterodimeric receptor complexes that transduce signals from the interferon, IL 10, and IL28 families of cytokines . IFN-./. R2, in association with IFN-./. R1, is required for mediating the antiviral,antiproliferative, and apoptotic effects of the type I interferons IFN-. and IFN-.. IFN-./. R2 is the principal ligand binding subunit of the receptor. Ligand binding is stabilized by the subsequent association with IFN-./. R1, resulting in the formation of a signaling ternary receptor complex . Mature human IFN-./. R2 consists of a 217 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, a 21 aa transmembrane segment, and a 251 aa cytoplasmic domain. Alternate splicing generates a secreted isoform that corresponds to the ECD and a 50 kDa transmembrane isoform with a substituted and truncated cytoplasmic region . The short isoform is impaired in its ability to activate signaling molecules and functions as a dominant negative receptor subunit . IFN-./. R2 is also subject to presenilin dependent intramembrane proteolysis, resulting in the liberation of nearly the entire ECD as well as the cytoplasmic domain which migrates to the nucleus and can inhibit gene transcription . High concentrations of soluble IFN-./. R2 bind and neutralize IFN-. and IFN-., while lower concentrations prolong the antiviral activity of circulating IFN-. but not IFN-. . Human but not mouse IFN-./. R2 constitutively associates with STAT4, which may account for species specific differences observed in type I interferon responses . Within the ECD, human IFN-./. R2 shares 63%, 60%, and 48% aa sequence identity with bovine, mouse, and ovine IFN-./. R2, respectively.
Gene Symbol	IFNA1
Standard Curve	Representative standard curve for IFN-a ELISA. IFN-a was diluted in serial two-fold steps in Sample Diluent.

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