Human IL-32a ELISA Kit (96-well)

Specifications

Product Data
Description	Human IL-32a ELISA Kit (96-well)
Size	1 x 96 wells
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	15.625-1000pg/ml
Sample Type	Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume	20 uL
Specificity	Natural and recombinant Human IL-32a Ligand
Sensitivity	7pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Aluminium pouches with a Microwell Plate coated with monoclonal antibody to human IL-32a(8x12) 2 vials human IL-32a Standard lyophilized, 500 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human IL-32a monoclonal antibody 2 vials Streptavidin-HRP solution 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 3 pieces Adhesive Films package insert
Background	IL-32. is the shortest and most abundant of four potential splice variants of the proinflammatory cytokine IL-32 (previously called NK4) with a predicted unmodified size of 15 kDa. Potential modifications include myristoylation and N-glycosylation. The IL-32. shows increased potency at inducing CXCL2/MIP-2 and CXCL8 expression in PBMC relative to uncleaved IL-32.. Transfected IL-32. was more likely to be cellassociated as compared to IL-32., suggesting an intracellular function. IL-32 is induced by mitogens in peripheral lymphocytes, by IFN. in epithelial cells, or by IL-12 with IL-18 in NK cells and in turn induces cytokine expression.
Gene Symbol	IL32
Standard Curve	Representative standard curve for IL-32a ELISA. IL-32a was diluted in serial two-fold steps in Sample Diluent.

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