Human Leptin ELISA Kit (96-well)

Specifications

Product Data
Description	Human Leptin ELISA Kit (96-well)
Size	1 x 96 wells
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	31.25-2000pg/ml
Sample Type	Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume	20 uL
Specificity	Natural and recombinantHuman Leptin Ligand
Sensitivity	7pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Aluminium pouches with a Microwell Plate coated with antibody to human Leptin (8x12) 2 vials human Leptin Standard lyophilized, 2000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human Leptin antibody 2 vials Streptavidin-HRP solution 4 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 4 pieces Adhesive Films package insert
Background	Human Leptin (gene name OB) is a 16 kDa, 146 amino acid (aa) residue, non-glycosylated polypeptide that regulates adipose tissue mass and energy balance . Mature human Leptin shares 87% and 84% aa identity with mouse and rat Leptin, respectively. Human Leptin is active in both the mouse and rat systems . Leptin is expressed almost exclusively by adipocytes and its production is influenced by hormones, cytokines and nutrients and circulates in the plasma, crosses the blood-brain barrier, and is present in human breast milk . The human Leptin receptor (designated ObR or LEPR) is a 150 kDa, 1144 aa residue, type I transmembrane glycoprotein of the IL-6 receptor family of Class I cytokine receptors . The gene for ObR undergoes considerable splicing, forming variants a-d with cytoplasmic domains of variable length, plus the potentially soluble form ObRe . The long form, ObRb (formerly OB RL), is expressed mainly in the hypothalamic arcuate nucleus and is essential for signal transduction . In a concentration-dependent manner, Leptin signaling can have diverse effects, causing neurons that express pro-opiomelanocortin (POMC) peptides to reduce food intake, and neurons that express neuropeptide Y and agouti-related protein (NpY and AgRP) to increase food intake . Leptin is fundamentally a Â?starvation signalÂ? that, when low, prompts increased appetite and decreased energy expenditure . Leptin deficiency influences the immune system, depressing Th1 responses and causing increased frequency of infections . Leptin also regulates puberty, blocking the onset of puberty, or of menses if Leptin deficiency exists due to excessive thinness, such as results from starvation, extreme exercise-induced weight loss, anorexia or cancer-induced cachexia .
Gene Symbol	LEP
Standard Curve	Representative standard curve for Leptin ELISA. Leptin was diluted in serial two-fold steps in Sample Diluent.

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