Human TGF-beta1 ELISA Kit (96-well)

Specifications

Product Data
Description	Human TGF-beta1 ELISA Kit (96-well)
Size	1 x 96 wells
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	62.5-4000pg/ml
Sample Type	Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume	20 uL
Specificity	Natural and recombinant Human TGF-β1 Ligand
Sensitivity	15pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Aluminium pouches with a Microwell Plate coated with antibody to human TGF-β1 (8x12) 2 vials human TGF-β1 Standard lyophilized, 2000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human TGF-β1 antibody 2 vials Streptavidin-HRP solution 4 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 1 vial 1N HCl 1 vial 1N NaOH 4 pieces Adhesive Films package insert
Background	Transforming growth factor (TGF), a Â?factorÂ? that promoted the transformation of cultured fibroblasts into a tumor-like phenotype, was subsequently found to be more of a tumor suppressor than tumor promoter and to be a mixture of two proteins, TGF-. and TGF-.. These molecules are members of a superfamily that includes TGF-.1 through 5, bone morphogenic proteins, activins and inhibins. It plays a critical role in cellular growth, development, differentiation, proliferation, extracellular matrix (ECM) synthesis and degradation, control of mesenchymal-epithelial interactions during embryogenesis, immune modulation, apoptosis, cell cycle progression, angiogenesis, adhesion and migration and leukocyte chemotaxis. Originally, TGF-.1 was separated from platelets and later found that TGF-.1 can be expressed in many organizations. Human TGF-.1 is a 25kDa, disulfide-linked, non-glycosylated homodimer. Biological activity of TGF-.1 is regulated by a number of receptors, including receptor I (53-65KD), receptor II (83-110KD), receptor III (250-310KD), receptor-IV (60KD) and receptor V (400KD). TGF-.1 is the key mediator in the pathophysiology of tissue repair and human fibrogenesis: balance between production and degradation of type I collagen, and fibrosis and scarring in organ and tissue. TGF-.1 exhibits important immunoregulatory features of partially adverse character: TGF-.1 inhibits B and T cell proliferation, differentiation and antibody production as well as maturation and activation of macrophages. TGF-.1 is synthesized, with only a few exceptions, by virtually all cells, and TGF receptors are expressed by all cells. TGF-. affects nearly every physiological process in some way; its systemic and cell-specific activities are too complicated to review here. There are, however, three fundamental activities: TGF-.1 modulates cell proliferation, generally as a suppressor; TGF-.1 enhances the deposition of extracellular matrix through promotion of synthesis and inhibition of degradation; TGF-.1 is immunosuppressive through a variety of mechanisms. The specific action of TGF-. on a particular cell depends on the exact circumstances of that cellÂ?s environment.
Gene Symbol	TGFB1
Standard Curve	Representative standard curve for TGF-?1 ELISA. TGF-?1 was diluted in serial two-fold steps in Sample Diluent.

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