Human GM-CSF ELISA Kit (96-well)

Specifications

Product Data
Description	Human GM-CSF ELISA Kit (96-well)
Size	1 x 96 wells
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	31.25-2000pg/ml
Sample Type	Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume	20 uL
Specificity	Natural and recombinant Human GM-CSF Ligand
Sensitivity	7pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Aluminium pouches with a Microwell Plate coated with monoclonal antibody to human GM-CSF (8x12) 2 vials human GM-CSF Standard lyophilized, 2000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human GM-CSF monoclonal antibody 2 vials Streptavidin-HRP solution 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 3 pieces Adhesive Films package insert
Background	Granulocyte macrophage colony stimulating factor (GM-CSF) is a pleiotropic cytokine with multiple effects on hematopoietic cells . It mobilizes CD34+ progenitor cells into the periphery and stimulates their proliferation, survival and differentiation into neutrophils,monocytes/ macrophages, eosinophils, and myeloid dendritic cells . On these terminallydifferentiated myeloid cells, GM-CSF is also needed for inducing their effector functions . In addition, GM-CSF has been shown to stimulate the proliferation and differentiation of the erythroid and megakaryoctye progenitor cells . GM-CSF is produced by a number of different cell types, including keratinocytes, mature and immature NK cells, type II alveolar cells), endothelial cells, monocytes, bone-marrow mesenchymal stem cells, CD4+ and CD8+ T cells, megakaryocytes, B cells, eosinophils, chondrocytes and fibroblasts. Human GM-CSF cDNA encodes a 144 amino acid (aa) residue precursor protein with a 17 aa putative signal peptide and a 127 aa mature protien . Natural GM-CSF is a monomer that contains both N- and O-linked glycosylation . Mature human GM-CSF shares approximately 55%, 63% and 68% aa sequence homology with mouse, rat and canine. GM-CSF, respectively. Human GM-CSF is not biologically active on mouse cells , but was reported to have some activity on canine cells . GM-CSF exerts its activity through binding to a high affinity receptor complex consisting of two membrane glycoproteins. The presence of the spliced variants in the heteromeric receptor complex can regulate the functions of the complex .
Gene Symbol	CSF2
Standard Curve	Representative standard curve for GM-CSF ELISA. GM-CSF was diluted in serial two-fold steps in Sample Diluent.

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